| Ribitol catabolic pathway in Klebsiella aerogenes. | |
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MedLine Citation:
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PMID: 4366025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In Klebsiella aerogenes W70, there is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (RDH) and d-ribulokinase (DRK). These two enzymes are coordinately controlled and induced in response to d-ribulose, an intermediate of the pathway. Whereas wild-type K. aerogenes W70 are unable to utilize xylitol as a carbon and energy source, mutants constitutive for the ribitol pathway are able to utilize RDH to oxidize the unusual pentitol, xylitol, to d-xylulose. These mutants are able to grow on xylitol, presumably by utilization of the d-xylulose produced. Mutants constitutive for l-fucose isomerase can utilize the isomerase to convert d-arabinose to d-ribulose. In the presence of d-ribulose, RDH and DRK are induced, and such mutants are thus able to phosphorylate the d-ribulose by using the DRK of the ribitol pathway. Derivatives of an l-fucose isomerase-constitutive mutant were plated on d-arabinose, ribitol, and xylitol to select and identify mutations in the ribitol pathway. Using the transducing phage PW52, we were able to demonstrate genetic linkage of the loci involved. Three-point crosses, using constitutive mutants as donors and RDH(-), DRK(-) double mutants as recipients and selecting for DRK(+) transductants on d-arabinose, resulted in DRK(+)RDH(+)-constitutive, DRK(+)RDH(+)-inducible, and DRK(+)RDH(-)-inducible transductants but no detectable DRK(+)RDH(-) constitutive transductants, data consistent with the order rbtC-rbtD-rbtK, where rbtC is a control site and rbtD and rbtK correspond to the sites for the sites for the enzymes RDH and DRK, respectively. |
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Authors:
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W T Charnetzky; R P Mortlock |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 119 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1974 Jul |
Date Detail:
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Created Date: 1974-09-11 Completed Date: 1974-09-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 162-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Oxidoreductases
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metabolism Arabinose / metabolism Carbohydrate Epimerases / metabolism Caseins Cell-Free System Chromosome Mapping Crosses, Genetic Culture Media Enterobacter / metabolism Enzyme Induction Fucose / metabolism Ketoses / metabolism Klebsiella / enzymology, growth & development, metabolism* Mutation Phosphotransferases / metabolism Ribose Stereoisomerism Sugar Alcohols / metabolism* Transduction, Genetic Xylitol / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Culture Media; 0/Ketoses; 0/Sugar Alcohols; 147-81-9/Arabinose; 3713-31-3/Fucose; 50-69-1/Ribose; 87-99-0/Xylitol; EC 1.1.-/Alcohol Oxidoreductases; EC 2.7.-/Phosphotransferases; EC 5.1.3.-/Carbohydrate Epimerases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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