Document Detail


Ribitol catabolic pathway in Klebsiella aerogenes.
MedLine Citation:
PMID:  4366025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Klebsiella aerogenes W70, there is an inducible pathway for the catabolism of ribitol consisting of at least two enzymes, ribitol dehydrogenase (RDH) and d-ribulokinase (DRK). These two enzymes are coordinately controlled and induced in response to d-ribulose, an intermediate of the pathway. Whereas wild-type K. aerogenes W70 are unable to utilize xylitol as a carbon and energy source, mutants constitutive for the ribitol pathway are able to utilize RDH to oxidize the unusual pentitol, xylitol, to d-xylulose. These mutants are able to grow on xylitol, presumably by utilization of the d-xylulose produced. Mutants constitutive for l-fucose isomerase can utilize the isomerase to convert d-arabinose to d-ribulose. In the presence of d-ribulose, RDH and DRK are induced, and such mutants are thus able to phosphorylate the d-ribulose by using the DRK of the ribitol pathway. Derivatives of an l-fucose isomerase-constitutive mutant were plated on d-arabinose, ribitol, and xylitol to select and identify mutations in the ribitol pathway. Using the transducing phage PW52, we were able to demonstrate genetic linkage of the loci involved. Three-point crosses, using constitutive mutants as donors and RDH(-), DRK(-) double mutants as recipients and selecting for DRK(+) transductants on d-arabinose, resulted in DRK(+)RDH(+)-constitutive, DRK(+)RDH(+)-inducible, and DRK(+)RDH(-)-inducible transductants but no detectable DRK(+)RDH(-) constitutive transductants, data consistent with the order rbtC-rbtD-rbtK, where rbtC is a control site and rbtD and rbtK correspond to the sites for the sites for the enzymes RDH and DRK, respectively.
Authors:
W T Charnetzky; R P Mortlock
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  119     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Jul 
Date Detail:
Created Date:  1974-09-11     Completed Date:  1974-09-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  162-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alcohol Oxidoreductases / metabolism
Arabinose / metabolism
Carbohydrate Epimerases / metabolism
Caseins
Cell-Free System
Chromosome Mapping
Crosses, Genetic
Culture Media
Enterobacter / metabolism
Enzyme Induction
Fucose / metabolism
Ketoses / metabolism
Klebsiella / enzymology,  growth & development,  metabolism*
Mutation
Phosphotransferases / metabolism
Ribose
Stereoisomerism
Sugar Alcohols / metabolism*
Transduction, Genetic
Xylitol / metabolism
Chemical
Reg. No./Substance:
0/Caseins; 0/Culture Media; 0/Ketoses; 0/Sugar Alcohols; 147-81-9/Arabinose; 3713-31-3/Fucose; 50-69-1/Ribose; 87-99-0/Xylitol; EC 1.1.-/Alcohol Oxidoreductases; EC 2.7.-/Phosphotransferases; EC 5.1.3.-/Carbohydrate Epimerases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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