Document Detail


Rhythms of glutamic acid decarboxylase mRNA in the suprachiasmatic nucleus.
MedLine Citation:
PMID:  8946258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the present study was to determine whether there is a rhythm in glutamic acid decarboxylase (GAD) message in the suprachiasmatic nucleus (SCN) of rats housed in a light:dark cycle. The mRNAs encoding two isoforms of GAD (i.e., GAD65 and GAD67) were examined using in situ hybridization histochemistry. Computerized image analysis of film autoradiographs revealed that GAD65 mRNA was significantly higher in the light than it was in the dark. GAD67 mRNA levels were lower overall and did not decrease significantly in the dark. Following emulsion autoradiography, silver grain counts over individual SCN cells indicated that GAD65 mRNA was highest in the dorsomedial hypothalamus during the light. These data suggest that GAD mRNA varies rhythmically in the SCN and that mRNA levels are regulated differently within SCN subdivisions during the light:dark cycle.
Authors:
K L Huhman; A C Hennessey; H E Albers
Related Documents :
17699798 - Regulation of genes of the circadian clock in human colon cancer: reduced period-1 and ...
24548428 - Expression of il-17a and il-17f in lipopolysaccharide-induced acute lung injury and the...
23056328 - Inhibition of notch signaling by a γ-secretase inhibitor attenuates hepatic fibrosis i...
1374498 - Nuclear mechanisms mediate rhythmic changes in vasopressin mrna expression in the rat s...
8131838 - Increase of c-jun mrna upon hypo-osmotic cell swelling of rat hepatoma cells.
11884428 - Thalidomide suppresses nf-kappa b activation induced by tnf and h2o2, but not that acti...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biological rhythms     Volume:  11     ISSN:  0748-7304     ISO Abbreviation:  J. Biol. Rhythms     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-02-28     Completed Date:  1997-02-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8700115     Medline TA:  J Biol Rhythms     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  311-6     Citation Subset:  IM; S    
Affiliation:
Department of Biology, Georgia State University, Atlanta 30303, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Base Sequence
Circadian Rhythm*
Darkness
Glutamate Decarboxylase / biosynthesis*
Isoenzymes / biosynthesis
Light
Male
Molecular Sequence Data
Oligonucleotide Probes
RNA, Messenger / biosynthesis
Rats
Rats, Sprague-Dawley
Sulfur Radioisotopes
Suprachiasmatic Nucleus / enzymology*
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
NS 30022/NS/NINDS NIH HHS; NS 34586/NS/NINDS NIH HHS; NS 34896/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Oligonucleotide Probes; 0/RNA, Messenger; 0/Sulfur Radioisotopes; EC 4.1.1.15/Glutamate Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reentrainment of motor activity and spontaneous neuronal activity in the suprachiasmatic nucleus of ...
Next Document:  A role for the circadian clock of the suprachiasmatic nuclei in the interpretation of serial melaton...