Document Detail


Rhythmic patterns in phagocytosis and the production of reactive oxygen species by zebrafish leukocytes.
MedLine Citation:
PMID:  18793741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multiple components of vertebrate immune systems have been shown to exhibit circadian fluctuations. While the zebrafish is currently generating a wealth of information on the molecular pacemakers that may control circadian rhythms, there have been no reports of rhythmic activity in zebrafish leukocytes. In this study, we found that phagocytosis and the production of reactive oxygen species by zebrafish leukocytes varied significantly throughout twenty-four hour periods. A distinct peak in cellular ROS levels occurred before dawn, while the kinetics of respiratory burst responses were least rapid at this time of day. Phagocytosis of E. coli peaked late in the day, whereas there was no daily variation in phagocytosis of S. aureus. As seen in other species, the number of bacteria ingested per cell peaked during the night. These data provide direct evidence of rhythmic immune system activity, and demonstrate that zebrafish can be a valuable model in which to study the relationships between circadian gene expression, systemic pacemakers, and the activity of vertebrate immune system cells.
Authors:
Jessica E Kaplan; Ryan D Chrenek; Joel G Morash; Catarina M Ruksznis; Lynn G Hannum
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-08-31
Journal Detail:
Title:  Comparative biochemistry and physiology. Part A, Molecular & integrative physiology     Volume:  151     ISSN:  1531-4332     ISO Abbreviation:  Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-11-03     Completed Date:  2009-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806096     Medline TA:  Comp Biochem Physiol A Mol Integr Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  726-30     Citation Subset:  IM    
Affiliation:
Department of Biology, Colby College, 5741 Mayflower Hill, Waterville, ME 04901, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Circadian Rhythm / immunology*
Escherichia coli / immunology
Female
Kidney / cytology,  immunology,  metabolism
Kinetics
Leukocytes / immunology,  metabolism
Male
Phagocytosis
Reactive Oxygen Species / metabolism
Respiratory Burst
Staphylococcus aureus / immunology
Zebrafish / immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
P20 RR-016463/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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