Document Detail

Rhythmic supplementation of 17beta-estradiol according to the physiological estrous cycle: effect on blood pressure in female ovariectomized rats.
MedLine Citation:
PMID:  19882501     Owner:  NLM     Status:  MEDLINE    
Female Wistar-Kyoto rats (WKY) show a 4-day estrous cycle. The aim of this study was to examine the impact of 17beta-estradiol supplementation every fourth day to ovariectomized rats - mimicking the physiological estrous cycle - on regulation of blood pressure. We monitored blood pressure telemetrically in intact females, ovariectomized (OVX), and ovariectomized WKY injected subcutaneously with 17beta-estradiol (OVX (E2)) in a 4-day rhythm for 24 weeks. Blood pressure decreased both in intact females and OVX (E2), whereas that of OVX persisted at constant levels. The underlying mechanisms studied include the nitric oxide pathway, the rennin-angiotensin system as well as the endothelin system. Serum and urinary nitrate/nitrite (NOx) as well as aortic eNOS decreased in OVX and were restored to normal in OVX (E2). Conversely, caveolin-1 was higher in OVX than in intact females and OVX (E2) while Hsp90 did not differ among groups. Plasma angiotensin II and aortic AT (1) receptor expression increased in OVX and were normalized in OVX (E2). AT (2) receptor expression was regulated reciprocally. Serum endothelin-1 was significantly elevated in OVX and OVX (E2). There was no difference in aortic ET (A) receptor expression between groups whereas ET (B) receptor expression was higher in intact females and OVX (E2) than in OVX. The study suggests that supplementation of 17beta-estradiol in female WKY according to the natural estrous cycle maintains the physiological blood pressure encompassing vasorelaxing and vasoconstricting pathways. The physiological estrous cycle should be kept in mind when cardiovascular data are to be collected/interpreted under estrogen supplementation.
A Grundt; C Grundt; K Knoth; B Lemmer
Publication Detail:
Type:  Journal Article     Date:  2009-10-30
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et m?tabolisme     Volume:  42     ISSN:  1439-4286     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-04     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  130-6     Citation Subset:  IM    
Copyright Information:
Georg Thieme Verlag KG Stuttgart * New York.
Institute of Experimental and Clinical Pharmacology and Toxicology, Ruprecht-Karls-Universit?t Heidelberg, Mannheim, Germany.
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MeSH Terms
Angiotensin II / metabolism
Blood Pressure / drug effects*
Endothelin-1 / metabolism
Estradiol / administration & dosage*,  blood,  urine
Estrous Cycle / drug effects*
Injections, Subcutaneous
Nitric Oxide Synthase Type III / metabolism
Rats, Inbred WKY
Receptor, Endothelin A / metabolism
Receptor, Endothelin B / metabolism
Renin-Angiotensin System / drug effects
Reg. No./Substance:
0/Endothelin-1; 0/Receptor, Endothelin A; 0/Receptor, Endothelin B; 11128-99-7/Angiotensin II; 50-28-2/Estradiol; EC protein, human; EC Oxide Synthase Type III

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