| Rhodopsin regeneration in the normal and in the detached/replaced retina of the skate. | |
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MedLine Citation:
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PMID: 1478278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bleaching and regeneration of rhodopsin in the skate retina was studied by means of fundus reflectometry, both in the normal eyecup preparation and after the retina had been detached and then replaced on the surface of the pigment epithelium (RPE). After bleaching virtually all the rhodopsin in the retinal test area of the normal eyecup, more than 90% of the photopigment was reformed after about 2 hr in darkness; over most of this time course, rhodopsin density rose linearly at a rate of 0.875% min-1 with a half-time of 55 min. Detaching the retina from its pigment epithelium resulted in a number of abnormalities, both structural and functional. Histological examination of the detached/replaced (D/R) retina showed striking alterations in the structural integrity of the RPE cells at their interface with the neural retina. The cells appeared vacuolated and misshapen, and the apical processes of the RPE, which normally ensheath the receptor outer segments, were shredded and free of their association with the visual cells. These morphological changes, as well as dilution of the IRBP content of the subretinal space caused by separation of the tissues, appear to be the main factors contributing to the functional abnormalities in rhodopsin kinetics. But despite these abnormalities and the persistent detachment, the rate of regeneration and the amount of rhodopsin reformed after bleaching were reduced by less than 50% of their normal values. The fact that a significant fraction of the bleached rhodopsin was regenerated under these conditions indicates that 11-cis retinal formed in the RPE was able to traverse a much greater than normal subretinal space to reach the opsin-bearing photoreceptor membranes. |
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Authors:
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Y Sun; H Ripps |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental eye research Volume: 55 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-02-09 Completed Date: 1993-02-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 679-89 Citation Subset: IM |
Affiliation:
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Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, Chicago. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Photic Stimulation Pigment Epithelium of Eye / anatomy & histology Retina / anatomy & histology, metabolism*, physiology Rhodopsin / metabolism* Skates (Fish) / anatomy & histology, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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EY-01792/EY/NEI NIH HHS; EY-06516/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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9009-81-8/Rhodopsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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