Document Detail


Rhodopseudomonas acidophila strain 10050 contains photosynthetic LH2 antenna complexes that are not enriched with phosphatidylglycerol, and the phospholipids have a fatty acyl composition that is unusual for purple non-sulfur bacteria.
MedLine Citation:
PMID:  12460683     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipid composition of Rhodopseudomonas acidophila strain 10050 grown aerobically or anaerobically in the light was determined. The major phospholipids present in the aerobic cells were phosphatidylethanolamine (PE; 54%), phosphatidylglycerol (PG; 24%) and cardiolipin (diphosphatidylglycerol, DPG) (14%), together with phosphatidylcholine (PC; 5%). On moving the cells to anaerobic photosynthetic growth in the light PE remained the major phospholipid (37-49%), but there was a major change in the proportion of PC, which increased to 31-33%, and corresponding reductions in the contents of PG to 11-16% and DPG to 4-5%. The fatty acid composition of the phospholipids was unusual, compared with other purple non-sulfur photosynthetic bacteria, in that it contained 16:0 (29%), 17:1 (20%) and 19:1 (9%) plus several mainly unsaturated 2-OH fatty acids (9% total) as major components, when grown aerobically in the dark. In contrast when grown photosynthetically under anaerobic conditions there was <2% 17:1 or 19:1 present, while the amounts of 16:1 and 18:1 increased, and 16:0 decreased. The phospholipid composition of the purified light-harvesting complex 2 (LH2) complex was PE (43%), PC (42%) and DPG (15%). Unexpectedly, there was no PG associated with the purified LH2. These findings contrast with previous studies on several other photosynthetic bacteria, which had shown an increase in PG upon photosynthetic growth [Biochem. J. 181 (1979) 339]. The prior hypothesis that phosphatidylglycerol has some specific role to play in the function of light-harvesting complexes cannot be true for Rps. acidophila. It is suggested that specific integral membrane proteins may strongly influence the phospholipid content of the host membranes into which they are inserted.
Authors:
Nicholas J Russell; Julie K Coleman; Tina D Howard; Evelyn Johnston; Richard J Cogdell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1556     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-03     Completed Date:  2003-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  247-53     Citation Subset:  IM    
Affiliation:
Department of Agricultural Sciences, Imperial College, Wye Campus, Ashford, Kent TN25 5AH, UK. nicholas.russell@ic.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry
Cell Fractionation
Cell Membrane / chemistry
Light
Macromolecular Substances
Phosphatidylglycerols / chemistry*
Phospholipids / chemistry*
Photosynthetic Reaction Center Complex Proteins / chemistry
Protein Structure, Quaternary
Rhodopseudomonas / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Macromolecular Substances; 0/Phosphatidylglycerols; 0/Phospholipids; 0/Photosynthetic Reaction Center Complex Proteins

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