| Rhodococcus fascians infection accelerates progression of tobacco BY-2 cells into mitosis through rapid changes in plant gene expression. | |
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MedLine Citation:
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PMID: 17547674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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* To characterize plant cell cycle activation following Rhodococcus fascians infection, bacterial impact on cell cycle progression of tobacco BY-2 cells was investigated. * S-phase-synchronized BY-2 cells were cocultivated with R. fascians and cell cycle progression was monitored by measuring mitotic index, cell cycle gene expression and flow cytometry parameters. Cell cycle alteration was further investigated by cDNA-AFLP (amplified fragment length polymorphism). * It was shown that cell cycle progression of BY-2 cells was accelerated only upon infection with bacteria whose virulence gene expression was induced by a leafy gall extract. Thirty-eight BY-2 genes showed a differential expression within 6 h post-infection. Among these, seven were previously associated with specific plant cell cycle phases (in particular S and G2/M phases). Several genes also showed a differential expression during leafy gall formation. * R. fascians-infected BY-2 cells provide a simple model to identify plant genes related to leafy gall development. R. fascians can also be regarded as a useful biotic agent to alter cell cycle progression and, thereby, gain a better understanding of cell cycle regulation in plants. |
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Authors:
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Olivier Vandeputte; Danny Vereecke; Adeline Mol; Marc Lenjou; Dirk Van Bockstaele; Mondher El Jaziri; Marie Baucher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The New phytologist Volume: 175 ISSN: 0028-646X ISO Abbreviation: New Phytol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-06-05 Completed Date: 2007-10-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882884 Medline TA: New Phytol Country: England |
Other Details:
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Languages: eng Pagination: 140-54 Citation Subset: IM |
Affiliation:
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Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, rue Adrienne Bolland 8, Gosselies, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Aphidicolin / metabolism Cell Cycle / genetics Cell Division Cell Line DNA, Complementary / genetics DNA, Plant / genetics Gene Expression Regulation, Plant* Kinetics Mitosis Mitotic Index Molecular Sequence Data Plant Diseases / microbiology* Plant Proteins / genetics Polymorphism, Genetic RNA, Messenger / genetics RNA, Plant / genetics Rhodococcus / pathogenicity* Sequence Alignment Sequence Homology, Amino Acid Tobacco / cytology, genetics*, growth & development, microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA, Plant; 0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Plant; 38966-21-1/Aphidicolin |
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