Document Detail

Rhodamine conjugates:specific and nonspecific binding properties in immunohistochemistry.
MedLine Citation:
PMID:  52316     Owner:  NLM     Status:  MEDLINE    
Anionic-exchange fractions of IgG labeled with FITC, MRITC, RB200SC, or RBITC were tested on different substrates, and the resultant fluorescence was evaluated with the Ploem optical system. Conjugations with MRITC or RB200SC were found to afford the following advantages over FITC: immunofluorescence sensitivity was elevated six to seven times on a molar basis; high sensitivity could be combined with a wide specificity interval; there was negligible fading of emitted light; there was negligible tissue autofluorescence at the excitation wavelength (546 nm); because of the two latter points, repeated observations could be made on tissue sections stored for several years; and eosinophilic leukocytes that are prone to yield nonspecific staining could easily by identified by switching to ultraviolet-blue light excitation.
P Brandtzaeg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  254     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1975 Jun 
Date Detail:
Created Date:  1975-12-12     Completed Date:  1975-12-12     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  35-54     Citation Subset:  IM    
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MeSH Terms
Antibodies / analysis
Antibody Specificity
Fluorescent Antibody Technique / methods*
Immunoglobulin A
Immunoglobulin Fab Fragments
Immunoglobulin G
Intestinal Mucosa / immunology
Microscopy, Fluorescence
Rhodamines* / standards*
Spleen / immunology
Staining and Labeling
Xanthenes* / standards*
Reg. No./Substance:
0/Antibodies; 0/Immunoglobulin A; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G; 0/Rhodamines; 0/Xanthenes; EC

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