| RhoC is essential for TGF-beta1-induced invasive capacity of rat ascites hepatoma cells. | |
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MedLine Citation:
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PMID: 16750170 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transforming growth factor-beta1 (TGF-beta1) is a multifunctional growth factor that plays a role in cell proliferation, differentiation, extracellular matrix production, apoptosis, and cell motility. We show here that TGF-beta1 increased the invasiveness of MM1 cells, which are a highly invasive clone of rat ascites hepatoma cells. Both mRNA and protein levels of RhoC but not RhoA in TGF-beta1-treated MM1 cells increased. In parallel with this increase in expression, RhoC activity was induced by TGF-beta1 treatment. When RhoC was overexpressed in MM1 cells, the invasive capacity increased. The RhoC-overexpressing cells formed more nodules than did mock cells when injected into rat peritoneum. Furthermore, when RhoC expression was reduced by transfection with shRNA/RhoC, the invasiveness of MM1 cells decreased with concomitant suppression of RhoC expression. Thus, the induced expression of RhoC by TGF-beta1 in MM1 cells plays a critical role in TGF-beta1-induced cell migration. |
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Authors:
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M Mukai; H Endo; T Iwasaki; M Tatsuta; A Togawa; H Nakamura; M Inoue |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-19 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 346 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-19 Completed Date: 2006-08-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 74-82 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537-8511, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Gene Expression Profiling Liver Neoplasms / pathology Liver Neoplasms, Experimental / pathology* Neoplasm Invasiveness Rats Transforming Growth Factor beta / physiology* Transforming Growth Factor beta1 rho GTP-Binding Proteins / genetics, physiology* rhoA GTP-Binding Protein / physiology |
| Chemical | |
Reg. No./Substance:
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0/TGFB1 protein, human; 0/Tgfb1 protein, rat; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; EC 3.6.5.2/rho GTP-Binding Proteins; EC 3.6.5.2/rhoA GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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