| The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 20974804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rho GTPases regulate multiple signaling pathways to control a number of cellular processes during epithelial morphogenesis. To investigate the downstream pathways through which Rho regulates epithelial apical junction formation, we screened a small interfering RNA (siRNA) library targeting 28 known Rho target proteins in 16HBE human bronchial epithelial cells. This led to the identification of the serine-threonine kinase PRK2 (protein kinase C-related kinase 2, also called PKN2). Depletion of PRK2 does not block the initial formation of primordial junctions at nascent cell-cell contacts but does prevent their maturation into apical junctions. PRK2 is recruited to primordial junctions, and this localization depends on its C2-like domain. Rho binding is essential for PRK2 function and also facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. We conclude that PRK2 is recruited to nascent cell-cell contacts through its C2-like and Rho-binding domains and promotes junctional maturation through a kinase-dependent pathway. |
| | |
Authors:
|
Sean W Wallace; Ana Magalhaes; Alan Hall |
Related Documents
:
|
15710384 - Ubiquitination of rhoa by smurf1 promotes neurite outgrowth. 21057634 - Rga4, a rho-gap from fission yeast: finding specificity within promiscuity. 12562894 - Protein kinase c is necessary for recovery from the thyrotropin-releasing hormone-induc... 17568564 - The small rho gtpase rac1 controls normal human dermal fibroblasts proliferation with p... 22193544 - Ww domain-containing oxidoreductase promotes neuronal differentiation via negative regu... 8853904 - Presence of physiologically stimulated ret in adult rat brain: induction of ret express... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-25 |
Journal Detail:
|
Title: Molecular and cellular biology Volume: 31 ISSN: 1098-5549 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-14 Completed Date: 2011-01-27 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 81-91 Citation Subset: IM |
Affiliation:
|
Cell Biology Program, Memorial Sloan-Kettering Cancer Center, 1274 York Ave., New York, NY 10065, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bronchi
/
cytology,
metabolism Cell Line Epithelial Cells / cytology, metabolism Humans Intercellular Junctions / metabolism* Protein Kinase C / antagonists & inhibitors, chemistry, genetics, metabolism* Protein Structure, Tertiary RNA, Small Interfering / genetics Signal Transduction rho GTP-Binding Proteins / antagonists & inhibitors, genetics, metabolism* rhoA GTP-Binding Protein / antagonists & inhibitors, genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
GM081435/GM/NIGMS NIH HHS; //Medical Research Council |
| Chemical | |
Reg. No./Substance:
|
0/RHOC protein, human; 0/RNA, Small Interfering; 124671-05-2/RHOA protein, human; EC 2.7.1.-/protein kinase N; EC 2.7.11.13/Protein Kinase C; EC 3.6.5.2/rho GTP-Binding Proteins; EC 3.6.5.2/rhoA GTP-Binding Protein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell p...
Next Document: Requirement for class II phosphoinositide 3-kinase C2alpha in maintenance of glomerular structure an...