| The Rho-family guanine nucleotide exchange factor GEFT enhances retinoic acid- and cAMP-induced neurite outgrowth. | |
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MedLine Citation:
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PMID: 16496360 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Rho GTPases are important regulators of neurite outgrowth and pathfinding. We have recently reported that a Rho-family guanine nucleotide exchange factor, GEFT, modulates dendrite spine morphology and basal neurite outgrowth in primary hippocampal neurons and Neuro2A cells, respectively. Here we demonstrate that GEFT protein is highly expressed in all regions of the brain and is highly up-regulated upon treatment of Neuro2A cells with retinoic acid and dibutyric cAMP, which promote dendrite and axon-like neurite extensions, respectively. Within retinoic acid-induced neurite extensions, GEFT is localized to actin-enriched regions in the primary neurites, with little or no expression from secondary branches. Dibutyric cAMP-induced neurite extensions are highly concentrated for GEFT at the actin-rich distal tip of the growth cone. Additionally, we demonstrate that GEFT promotes neurite outgrowth in undifferentiated as well as differentiated Neuro2A cells. Together, our data provide new evidence suggesting that GEFT is an important regulator of multiple processes involved in axon and dendrite formation. |
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Authors:
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Brad A Bryan; Yi Cai; Mingyao Liu |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience research Volume: 83 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-04-26 Completed Date: 2006-07-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 1151-9 Citation Subset: IM |
Copyright Information:
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Copyright 2006 Wiley-Liss, Inc. |
Affiliation:
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Alkek Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Brain / cytology, embryology*, metabolism Bucladesine / pharmacology Cell Differentiation / drug effects, physiology* Cell Line, Tumor Cyclic AMP / metabolism*, pharmacology Dendrites / drug effects, metabolism, ultrastructure Gene Expression Regulation, Developmental / physiology Growth Cones / drug effects, metabolism, ultrastructure Guanine Nucleotide Exchange Factors / metabolism* Mice Neurites / drug effects, metabolism*, ultrastructure Rats Transfection Tretinoin / metabolism*, pharmacology Up-Regulation / drug effects, physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01HL64792/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/GEFT protein, human; 0/Guanine Nucleotide Exchange Factors; 302-79-4/Tretinoin; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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