Document Detail

Rho GTPases in alveolar macrophage phagocytosis.
MedLine Citation:
PMID:  18374173     Owner:  NLM     Status:  MEDLINE    
Alveolar macrophages are "professional phagocytes" and critical effector cells that protect the lungs from a broad array of microbes that can cause severe respiratory tract infections such as pneumonia. The molecular mechanisms that mediate microbial phagocytosis in alveolar macrophages are not fully known, and the specific role of small Rho GTPases has not been established. Most studies of Rho GTPase and phagocytosis focus on cell lines and transfected cells, and results may not accurately represent mechanisms operant in the lungs of humans. The use of clinically relevant primary human lung macrophages to examine phagocytosis in the context of host defense function may provide data that translate more readily to human conditions in health and disease. This chapter provides a description of methods and techniques for isolating, culturing, and assaying human alveolar macrophages for studies of small Rho GTPases and phagocytosis in the context of host defense. Data support the concept that different macrophage phagocytic receptors may exhibit distinct molecular mechanisms of small Rho GTPase activation that mediate phagocytosis.
Henry Koziel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in enzymology     Volume:  439     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-31     Completed Date:  2008-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  303-13     Citation Subset:  IM    
Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
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MeSH Terms
Bronchoalveolar Lavage Fluid / cytology
Macrophages, Alveolar / physiology*
Middle Aged
Phagocytosis / physiology*
cdc42 GTP-Binding Protein / physiology
rac1 GTP-Binding Protein / physiology
rho GTP-Binding Proteins / physiology*
Grant Support
Reg. No./Substance:
EC GTP-Binding Protein; EC GTP-Binding Protein; EC GTP-Binding Proteins

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