| Rho GTPase-mediated cytoskeletal organization in Schlemm's canal cells play a critical role in the regulation of aqueous humor outflow facility. | |
| | |
MedLine Citation:
|
PMID: 21268081 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The increased intraocular pressure (IOP) has been considered to be an increased resistance of the aqueous humor outflow through the inner wall of Schlemm's canal (SC) and/or the juxtacanalicular tissue (JCT). The Rho GTPase-regulated actomyosin organization appears to be an important mechanistic determinant of aqueous humor outflow facility. Therefore, in this study, we have evaluated the effects of modulating Rho GTPase activity on actomyosin cytoskeletal organization, monolayer permeability/barrier function of human SC cells, and aqueous humor outflow facility in enucleated porcine eyes ex vivo. Human SC cells, isolated from cadaver eyes, were treated with either Rho GTPase activators such as thrombin and lysophosphatidic acid (LPA), or a specific inhibitor (C3-exoenzyme) of Rho GTPases. Treatment of SC cells with thrombin and LPA led to increased formation of stress fibers, focal adhesion, and increased myosin light chain phosphorylation, whereas treatment with C3-exoenzyme showed the opposite effects like H-7 and ECA, known for increasing the outflow facility in porcine eyes. The findings presented here suggest that LPA and thrombin, presumably through activation of Rho GTPase-mediated actomyosin cytoskeletal reorganization in SC cells, cause a decrease in monolayer permeability of SC cells as well as a decrease in outflow facility of porcine eyes in ex vivo. Our results suggest that decrease in aqueous humor outflow may be correlated better with the changes in cytoskeletal organizations of SC, which could be the prime locus of the outflow resistance. |
| | |
Authors:
|
Janardan Kumar; David L Epstein |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 112 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-01-26 Completed Date: 2011-05-03 Revised Date: 2013-05-02 |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 600-6 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Wiley-Liss, Inc. |
Affiliation:
|
Department of Natural Sciences, Becker College, Worcester, Massachusetts 01609, USA. janardan.kumar@becker.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actins
/
metabolism Aqueous Humor / cytology*, physiology Cells, Cultured Cytoskeleton / metabolism* Endothelial Cells / metabolism* Focal Adhesions / metabolism Humans Myosin Light Chains / metabolism Phosphorylation Thrombin / metabolism rho GTP-Binding Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 EY001894/EY/NEI NIH HHS; R01 EY01894/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/Myosin Light Chains; EC 3.4.21.5/Thrombin; EC 3.6.5.2/rho GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cigarette smoke extract regulates cytosolic phospholipase A(2) expression via NADPH oxidase/MAPKs/AP...
Next Document: Changes in matrix protein gene expression associated with mineralization in the differentiating chic...