Document Detail


A Rhizobium meliloti symbiotic regulatory gene.
MedLine Citation:
PMID:  6423287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have characterized a Rhizobium meliloti regulatory gene required for the expression of two closely linked symbiotic operons, the nitrogenase operon (nifHDK genes) and the "P2" operon. This regulatory gene maps to a 1.8 kb region located 5.5 kb upstream of the nifHDK operon. The regulatory gene is required for the accumulation of nifHDK and P2 mRNA and for the derepression of an R. meliloti nifH-lacZ fusion plasmid during symbiotic growth. The nifH and P2 promoters can be activated in free-living cultures of R. meliloti containing plasmids that produce the Escherichia coli ntrC(glnG) or the Klebsiella pneumoniae nifA regulatory gene products constitutively. The R. meliloti regulatory gene hybridizes to E. coli ntrC(glnG) and, to a lesser extent, to K. pneumoniae nifA DNA. Our results suggest that the R. meliloti regulatory gene acts as a positive transcriptional activator and that it is related to the K. pneumoniae nif regulatory genes.
Authors:
W W Szeto; J L Zimmerman; V Sundaresan; F M Ausubel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  36     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-05-24     Completed Date:  1984-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1035-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / genetics
Genes
Genes, Bacterial*
Genes, Regulator*
Klebsiella pneumoniae / genetics
Linkage (Genetics)
Medicago sativa / growth & development
Nitrogenase / genetics
Nucleic Acid Hybridization
Operon
Plants / growth & development
Plasmids
Rhizobium / genetics*,  physiology
Symbiosis*
beta-Galactosidase / analysis
Chemical
Reg. No./Substance:
EC 1.18.6.1/Nitrogenase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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