Document Detail

Rhizobium leguminosarum chaperonin 60.3, but not chaperonin 60.1, induces cytokine production by human monocytes: activity is dependent on interaction with cell surface CD14.
MedLine Citation:
PMID:  12380680     Owner:  NLM     Status:  MEDLINE    
As part of a program of work to understand the interaction of bacterial chaperonins with human leukocytes, we have examined 2 of the 3 chaperonin 60 (Cpn 60) gene products of the nonpathogenic plant symbiotic bacterium, Rhizobium leguminosarum, for their capacity to induce the production of pro- and antiinflammatory cytokines by human cells. Recombinant R. leguminosarum Cpn 60.1 and 60.3 proteins were added to human monocytes at a range of concentrations, and cytokine production was measured by sandwich enzyme-linked immunosorbent assay. In spite of the fact that the 2 R. leguminosarum Cpn 60 proteins share 74.5% amino acid sequence identity, it was found that Cpn 60.3 induced the production of interleukin (IL)-1beta, tumor necrosis factor alpha, IL-6, IL-8, IL-10, and IL-12, but not IL-4, interferon gamma, or GM-CSF (granulocyte-macrophage colony-stimulating factor), whereas the Cpn 60.1 protein failed to demonstrate any cytokine-inducing activity. The use of neutralizing monoclonal antibodies showed that the cytokine-inducing activity of Cpn 60.3 was dependent on its interaction with CD14. This demonstrates that CD14 mediates not only lipopolysaccharide but also R. leguminosarum Cpn 60.3 cell signaling in human monocytes.
Jo Lewthwaite; Roger George; Peter A Lund; Steve Poole; Peter Tormay; Lindsay Sharp; Anthony R M Coates; Brian Henderson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell stress & chaperones     Volume:  7     ISSN:  1355-8145     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-10-16     Completed Date:  2003-02-12     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-6     Citation Subset:  IM    
Cellular Microbiology Research Group, Eastman Dental Institute, University College London, UK.
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MeSH Terms
Amino Acid Sequence
Antigens, CD14 / metabolism*
Bacterial Proteins / genetics,  metabolism*
Chaperonin 60 / genetics,  metabolism*
Cytokines / biosynthesis*
Endotoxins / metabolism
Flagellin / metabolism
Molecular Sequence Data
Monocytes / metabolism*,  microbiology
Polymyxin B / metabolism
Rhizobium leguminosarum / metabolism*
Sequence Alignment
Reg. No./Substance:
0/Antigens, CD14; 0/Bacterial Proteins; 0/Chaperonin 60; 0/Cytokines; 0/Endotoxins; 12777-81-0/Flagellin; 1404-26-8/Polymyxin B

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