Document Detail


Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling.
MedLine Citation:
PMID:  23136382     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nodulation in legumes requires the recognition of rhizobially made Nod factors. Genetic studies have revealed that the perception of Nod factors involves LysM domain receptor-like kinases, while biochemical approaches have identified LECTIN NUCLEOTIDE PHOSPHOHYDROLASE (LNP) as a Nod factor-binding protein. Here, we show that antisense inhibition of LNP blocks nodulation in Lotus japonicus. This absence of nodulation was due to a defect in Nod factor signaling based on the observations that the early nodulation gene NODULE INCEPTION was not induced and that both Nod factor-induced perinuclear calcium spiking and calcium influx at the root hair tip were blocked. However, Nod factor did induce root hair deformation in the LNP antisense lines. LNP is also required for infection by the mycorrhizal fungus Glomus intraradices, suggesting that LNP plays a role in the common signaling pathway shared by the rhizobial and mycorrhizal symbioses. Taken together, these observations indicate that LNP acts at a novel position in the early stages of symbiosis signaling. We propose that LNP functions at the earliest stage of the common nodulation and mycorrhization symbiosis signaling pathway downstream of the Nod factor receptors; it may act either by influencing signaling via changes in external nucleotides or in conjunction with the LysM receptor-like kinases for recognition of Nod factor.
Authors:
Nicholas J Roberts; Giulia Morieri; Gurpreet Kalsi; Alan Rose; Jiri Stiller; Anne Edwards; Fang Xie; Peter M Gresshoff; Giles E D Oldroyd; J Allan Downie; Marilynn E Etzler
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-07
Journal Detail:
Title:  Plant physiology     Volume:  161     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-06-18     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  556-67     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Apyrase / genetics,  metabolism*
Calcium / metabolism
Calcium Signaling*
Gene Knockdown Techniques
Lipopolysaccharides / metabolism
Lotus / enzymology,  genetics,  microbiology*
Mesorhizobium / growth & development*
Mycorrhizae / growth & development*,  metabolism
Nitrogen Fixation
Phenotype
Plant Proteins / genetics,  metabolism
Plant Root Nodulation
Plants, Genetically Modified / microbiology
Root Nodules, Plant / metabolism,  microbiology
Symbiosis*
Grant Support
ID/Acronym/Agency:
GM21882/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/NIN protein, Lotus japonicus; 0/Plant Proteins; 7440-70-2/Calcium; EC 3.6.1.5/Apyrase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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