Document Detail


Rhein inhibits renal tubular epithelial cell hypertrophy and extracellular matrix accumulation induced by transforming growth factor beta1.
MedLine Citation:
PMID:  11749778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effects of rhein on cell hypertrophy and accumulation of extracellular matrix (ECM) in the renal tubular epithelial cells. METHODS: LLC-PK1 cells were incubated with transforming growth factor beta1 (TGFbeta1) 2 microg/L for 24 h to induce cell hypertrophy and production of ECM. To evaluate the effects of rhein on inhibiting the action of TGFbeta1, cell volume, cellular protein level, and [3H]leucine incorporation in LLC-PK1 cells treated with rhein at different concentrations were measured. In addition, the [3H]proline incorporation, level of fibronectin (FN) in supernatant, and mRNA expression of collagen IV and FN were also detected in rhein treated cells. RESULTS: The cell volume, cellular protein content, and [3H]leucine incorporation were markedly increased in LLC-PK1 cells after TGFbeta1 stimulation as compared with control (P < 0.01), and this TGFbeta1-stimulated cell hypertrophy was ameliorated by rhein. It was observed that TGFbeta1 not only increased the production of FN and [3H]proline incorporation in LLC-PK1 cells (P < 0.01), but also enhanced the mRNA expression of collagen IV and FN. Rhein significantly decreased the protein production and mRNA expression of ECM in LLC-PK1 cells stimulated by TGFbeta1. CONCLUSION: Rhein can inhibit cell hypertrophy and ECM accumulation in LLC-PK1 cells induced by TGFbeta1, which may partly account for the role of rhein in preventing and retarding the progression of diabetic nephropathy.
Authors:
X H Guo; Z H Liu; C S Dai; H Li; D Liu; L S Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  22     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2003-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  934-8     Citation Subset:  IM    
Affiliation:
Research Institute of Nephrology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anthraquinones / pharmacology*
Collagen Type IV / biosynthesis*,  genetics
Epithelial Cells / pathology
Extracellular Matrix / metabolism
Fibronectins / biosynthesis,  genetics
Hypertrophy
Kidney Tubules, Proximal / pathology*
RNA, Messenger / biosynthesis,  genetics
Swine
Transforming Growth Factor beta / antagonists & inhibitors,  pharmacology*
Transforming Growth Factor beta1
Chemical
Reg. No./Substance:
0/Anthraquinones; 0/Collagen Type IV; 0/Fibronectins; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 478-43-3/rhein

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