| Rhamnolipid-producing thermophilic bacteria of species Thermus and Meiothermus. | |
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MedLine Citation:
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PMID: 21984420 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Novel rhamnolipid-producing strains of three thermophilic bacteria, Thermus sp., T. aquaticus and Meiothermus ruber were identified that have not been previously described as rhamnolipid producers. Rhamnolipids were extracted from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed 77 rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homologues containing one or two 3-hydroxy-fatty acids, saturated, monounsaturated or diunsaturated, even- or odd-chain, up to unusual long chains with 24 carbon atoms. The stereochemistry of rhamnose was L and that of 3-hydroxy-fatty acids was R, the position of double bonds in monoenoic acids was cis ω-9. All three strains produced a rhamnolipid that differs in structure from Pseudomonas aeruginosa rhamnolipids and exhibits excellent surfactant properties. Importantly, in comparison to P. aeruginosa both strains, i.e., Thermus and Meiothermus, are Biosafety level 1 microorganisms and are not pathogenic to humans. |
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Authors:
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Tomáš Rezanka; Lucie Siristova; Karel Sigler |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-9 |
Journal Detail:
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Title: Extremophiles : life under extreme conditions Volume: - ISSN: 1433-4909 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706854 Medline TA: Extremophiles Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic, rezanka@biomed.cas.cz. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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