| Rhabdastrellic acid-A inhibited PI3K/Akt pathway and induced apoptosis in human leukemia HL-60 cells. | |
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MedLine Citation:
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PMID: 17920303 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Increasing evidence suggests that aberrant activation of PI3K/Akt is involved in many human cancers, and that inhibition of the PI3K/Akt pathway might be a promising strategy for cancer treatment. Our investigation indicates that Rhabdastrellic acid-A, an isomalabaricane triterpenoid isolated from the sponge, Rhabdastrella globostellata, inhibits proliferation of HL-60 cells with an IC(50) value of 0.68mug/ml, and induces apoptosis. Rhabdastrellic acid-A also induces cleavage of the death substrate poly (ADP-ribose) polymerase (PARP) and caspase-3. Pretreatment of HL-60 cells with the caspase-3 specific inhibitor, DEVD-CHO, prevents Rhabdastrellic acid-A-induced DNA fragmentation and PARP cleavage. Activated PI3K and Akt significantly decreases after treatment with Rhabdastrellic acid-A in HL-60 cells. Expression levels of protein bcl-2, bax remain unchanged in response to Rhabdastrellic acid-A treatment in HL-60 cells. These results suggest that Rhabdastrellic acid-A inhibits PI3K/Akt pathway and induces caspase-3 dependent-apoptosis in HL-60 human leukemia cells. |
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Authors:
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Jing-Feng Guo; Jun-Min Zhou; Yong Zhang; Rong Deng; Jian-Nan Liu; Gong-Kan Feng; Zong-Chao Liu; Ding-Jun Xiao; Song-Zhi Deng; Xiao-Feng Zhu |
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Publication Detail:
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Type: Journal Article Date: 2007-09-01 |
Journal Detail:
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Title: Cell biology international Volume: 32 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-02-22 Completed Date: 2008-06-12 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 48-54 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, 651 Dongfeng Road East, Guangzhou 510060, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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antagonists & inhibitors* Apoptosis / drug effects* Caspase 3 / antagonists & inhibitors, metabolism DNA Damage Down-Regulation Enzyme Activation HL-60 Cells Humans Oligopeptides / pharmacology Proto-Oncogene Proteins c-akt / antagonists & inhibitors* Proto-Oncogene Proteins c-bcl-2 / biosynthesis Signal Transduction / drug effects* Triterpenes / pharmacology* bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/BAX protein, human; 0/Oligopeptides; 0/Proto-Oncogene Proteins c-bcl-2; 0/Triterpenes; 0/aspartyl-glutamyl-valyl-aspartal; 0/bcl-2-Associated X Protein; 0/rhabdastrellic acid A; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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