Document Detail

Rg1 protects iron-induced neurotoxicity through antioxidant and iron regulatory proteins in 6-OHDA-treated MES23.5 cells.
MedLine Citation:
PMID:  20872781     Owner:  NLM     Status:  In-Process    
Ginsenoside-Rg1 is one of the pharmacologically active components isolated from ginseng. It was reported that Rg1 protected dopamine (DA) neurons in 6-hydroxydopamine (6-OHDA)-induced Parkinson's disease (PD) models in vivo and in vitro. Our previous study also demonstrated that iron accumulation was involved in the toxicity of 6-OHDA. However, whether Rg1 could protect DA neurons against 6-OHDA toxicity by modulating iron accumulation and iron-induced oxidative stress is not clear. Therefore, the present study was carried out to elucidate this effect in 6-OHDA-treated MES23.5 cells and the possible mechanisms were also conducted. Findings showed Rg1 restored iron-induced decrease in mitochondrial transmembrane potential in MES23.5 cells, and increased ferrous iron influx was found in 6-OHDA-treated cells. Rg1 pretreatment could decrease this iron influx by inhibiting 6-OHDA-induced up-regulation of an iron importer protein divalent metal transporter 1 with iron responsive element (DMT1 + IRE). Furthermore, findings also showed that the effect of Rg1 on DMT1 + IRE expression was due to its inhibition of iron regulatory proteins (IRPs) by its antioxidant effect. These results suggested that the neuroprotective effect of Rg1 against iron toxicity in 6-OHDA-treated cells was to decrease the cellular iron accumulation and attenuate the improper up-regulation of DMT1 + IRE via IRE/IRP system. This provides new insight to understand the pharmacological effects of Rg1 on iron-induced degeneration of DA neurons.
Huamin Xu; Hong Jiang; Jun Wang; Junxia Xie
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  111     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1537-45     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines Physiology, Medical College of Qingdao University, Qingdao 266071, China.
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