Document Detail


Rewritable DNA microarrays.
MedLine Citation:
PMID:  16478182     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thiol-terminated single-stranded deoxyribonucleic acids (ssDNA) can be immobilized onto pulsed plasma deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily undergo nucleic acid hybridization. Unlike other methods for oligonucleotide attachment to solid surfaces, this approach is shown to be independent of substrate material or geometry, and amenable to highly efficient rewriting.
Authors:
W C E Schofield; J McGettrick; T J Bradley; J P S Badyal; S Przyborski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  128     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-15     Completed Date:  2006-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2280-5     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Science Laboratories, and School of Biological and Biomedical Science, Durham University, Durham DH1 3LE, England, UK.
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MeSH Terms
Descriptor/Qualifier:
DNA, Single-Stranded / chemistry*
Disulfides / chemistry*
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis / methods*
Oligonucleotide Probes / chemistry
Spectroscopy, Fourier Transform Infrared
Sulfhydryl Compounds / chemistry
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Disulfides; 0/Oligonucleotide Probes; 0/Sulfhydryl Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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