| A review of bioreactor protocols for human neural precursor cell expansion in preparation for clinical trials. | |
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MedLine Citation:
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PMID: 21476982 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tissue-specific human neural precursor cells (hNPCs) can be isolated from various regions of the developing or adult central nervous system and may serve as a viable source of cells in cell replacement therapies for the treatment of neurodegenerative disorders. However, in order for cell replacement strategies to become a routine therapeutic option for the treatment of neurodegenerative disorders, hNPCs should be generated under standardized and controlled conditions. Studies over the last two decades have focused on developing cell growth media and cell handling protocols for expansion and differentiation of hNPCs in culture. Key studies have reported the development of serum-free growth media and large-scale computer-controlled suspension bioreactors that can support high cell proliferation rates (doubling times < 3 days), multipotentiality, and potential neurogenic differentiation (more than 60% neurons). Moreover, bioengineering studies have focused on controlling culture conditions in suspension bioreactors including inoculation, hydrodynamics of culture, oxygen and nutrients transfer to the cells, monitoring in situ physiological parameters using process control techniques, and expansion for extended periods of time. In addition, in vitro and in vivo characterization of hNPCs have been performed, providing information on stem/progenitor cell characteristics, cell surface analysis, and appropriate type of cells to use in transplantation studies. |
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Authors:
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Behnam A Baghbaderani; Karim Mukhida; Murray Hong; Ivar Mendez; Leo A Behie |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Current stem cell research & therapy Volume: 6 ISSN: 1574-888X ISO Abbreviation: Curr Stem Cell Res Ther Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-10 Completed Date: 2012-01-09 Revised Date: 2012-01-19 |
Medline Journal Info:
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Nlm Unique ID: 101272517 Medline TA: Curr Stem Cell Res Ther Country: United Arab Emirates |
Other Details:
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Languages: eng Pagination: 229-54 Citation Subset: IM |
Affiliation:
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Schulich School of Engineering, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Surface / metabolism Batch Cell Culture Techniques / instrumentation, methods* Bioreactors* Cell Differentiation Clinical Trials as Topic / instrumentation, methods* Culture Media Drug Delivery Systems Humans Neural Stem Cells / cytology*, metabolism, physiology, transplantation Neurodegenerative Diseases / therapy Time-Lapse Imaging |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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