Document Detail

A review of bioreactor protocols for human neural precursor cell expansion in preparation for clinical trials.
MedLine Citation:
PMID:  21476982     Owner:  NLM     Status:  MEDLINE    
Tissue-specific human neural precursor cells (hNPCs) can be isolated from various regions of the developing or adult central nervous system and may serve as a viable source of cells in cell replacement therapies for the treatment of neurodegenerative disorders. However, in order for cell replacement strategies to become a routine therapeutic option for the treatment of neurodegenerative disorders, hNPCs should be generated under standardized and controlled conditions. Studies over the last two decades have focused on developing cell growth media and cell handling protocols for expansion and differentiation of hNPCs in culture. Key studies have reported the development of serum-free growth media and large-scale computer-controlled suspension bioreactors that can support high cell proliferation rates (doubling times < 3 days), multipotentiality, and potential neurogenic differentiation (more than 60% neurons). Moreover, bioengineering studies have focused on controlling culture conditions in suspension bioreactors including inoculation, hydrodynamics of culture, oxygen and nutrients transfer to the cells, monitoring in situ physiological parameters using process control techniques, and expansion for extended periods of time. In addition, in vitro and in vivo characterization of hNPCs have been performed, providing information on stem/progenitor cell characteristics, cell surface analysis, and appropriate type of cells to use in transplantation studies.
Behnam A Baghbaderani; Karim Mukhida; Murray Hong; Ivar Mendez; Leo A Behie
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current stem cell research & therapy     Volume:  6     ISSN:  1574-888X     ISO Abbreviation:  Curr Stem Cell Res Ther     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-10     Completed Date:  2012-01-09     Revised Date:  2012-01-19    
Medline Journal Info:
Nlm Unique ID:  101272517     Medline TA:  Curr Stem Cell Res Ther     Country:  United Arab Emirates    
Other Details:
Languages:  eng     Pagination:  229-54     Citation Subset:  IM    
Schulich School of Engineering, University of Calgary, Alberta, Canada.
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MeSH Terms
Antigens, Surface / metabolism
Batch Cell Culture Techniques / instrumentation,  methods*
Cell Differentiation
Clinical Trials as Topic / instrumentation,  methods*
Culture Media
Drug Delivery Systems
Neural Stem Cells / cytology*,  metabolism,  physiology,  transplantation
Neurodegenerative Diseases / therapy
Time-Lapse Imaging
Reg. No./Substance:
0/Antigens, Surface; 0/Culture Media

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