| Reversion of the invasive phenotype of transformed human fibroblasts by anti-messenger oligonucleotide inhibition of urokinase receptor gene expression. | |
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MedLine Citation:
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PMID: 7805047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The receptors for urokinase plasminogen activator were studied in both normal human fibroblasts (WI-38 cells) and their SV40-transformed counterpart (VA-13 cells). We have shown that transformed cells expose 10 times more urokinase plasminogen activator receptors (u-PAR) than normal cells. By cross-linking aliquots of cell lysates with the aminoterminal fragment of the A chain of u-PA, containing the receptor-binding sequence, we have observed a u-PAR concentration at focal contacts in both cell lines. Only transformed cells were able to efficiently invade the basement membrane Matrigel. Switching off the receptor gene expression by the anti-messenger oligodeoxynucleotides strategy abolished the invasive properties of transformed cells. The anti-messenger oligodeoxynucleotide sequence we have designed inhibited the u-PAR gene expression, lowering both the receptor and the receptor mRNA. This indicates that overexpression of u-PAR gene is itself responsible for invasivity of transformed fibroblasts in our cell model system and that antisense compound therapy may prove to be of clinical interest in the control of cancer spreading. |
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Authors:
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A Quattrone; G Fibbi; E Anichini; M Pucci; A Zamperini; S Capaccioli; M Del Rosso |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 55 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-01-30 Completed Date: 1995-01-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 90-5 Citation Subset: IM |
Affiliation:
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Istituto di Patologia Generale, Università di Firenze, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Transformation, Viral Cells, Cultured Fibroblasts Humans Molecular Sequence Data Neoplasm Invasiveness* Oligonucleotides, Antisense / pharmacology* Plasminogen Activators / metabolism Receptors, Cell Surface / metabolism* Receptors, Urokinase Plasminogen Activator Simian virus 40 Urokinase-Type Plasminogen Activator / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides, Antisense; 0/PLAUR protein, human; 0/Receptors, Cell Surface; 0/Receptors, Urokinase Plasminogen Activator; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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