| Reversible transformation by urea of contact-inhibited fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 4509335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
200 mM urea elicits alterations of cell social behavior in vitro. In the presence of urea, contact inhibition of movement and growth is reduced compared to untreated fibroblastic cell populations. This reduction of contact inhibition is rapidly reversible, but reversal requires some cycloheximide-sensitive cellular process. Cells treated with urea are agglutinable by concanavalin A, suggesting that urea might remove some cell-surface component(s). A nondialyzable factor can, in fact, be detected in the supernatant medium of urea-treated cells. This active constituent appears to be heat-labile and trypsin-sensitive, and can itself restore normal contact-inhibitory behavior to cells maintained in the urea-transformed state by cycloheximide. This system may permit identification and characterization of surface components that are involved in regulating normal cell social behavior. |
| | |
Authors:
|
J A Weston; K L Hendricks |
Related Documents
:
|
227995 - Loss of proviral dna sequences in a revertant of kirsten sarcoma virus-transformed muri... 3098905 - Genetic transformation of rifampicin resistance in lactobacillus acidophilus. 9168445 - In vitro culture studies of childhood myelodysplastic syndrome: establishment of the ce... 3587255 - Comparative analysis of dna loop length in nontransformed and transformed hamster cells. 21935475 - A new extensively characterised conditionally immortal muscle cell-line for investigati... 17553915 - Kinetic studies of 25-hydroxy-19-nor-vitamin d3 and 1 alpha,25-dihydroxy-19-nor-vitamin... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 69 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1972 Dec |
Date Detail:
|
Created Date: 1973-03-02 Completed Date: 1973-03-02 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3727-31 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agglutination
/
drug effects Cell Division Cell Line Concanavalin A / pharmacology Contact Inhibition / drug effects* Culture Media Cycloheximide / pharmacology Fibroblasts / drug effects, metabolism Lysine / metabolism Myocardium Tritium Urea / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 10028-17-8/Tritium; 11028-71-0/Concanavalin A; 56-87-1/Lysine; 57-13-6/Urea; 66-81-9/Cycloheximide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Alkylamine-dependent amino-acid oxidation by lysine monooxygenase--fragmented substrate of oxygenase...
Next Document: Hapten-conjugated antibodies and visual markers used to label cell-surface antigens for electron mic...