Document Detail


Reversible tracheal obstruction in the fetal sheep: effects on tracheal fluid pressure and lung growth.
MedLine Citation:
PMID:  7472976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Congenital diaphragmatic hernia (CDH) continues to carry high morbidity and mortality. A number of treatment modalities including extracorporeal membrane oxygenation and in utero repair have improved the mortality rate only minimally. With this condition, there is often insufficient lung mass at birth and persistent pulmonary hypertension postnatally. Experimental fetal tracheal ligation has been shown to increase lung growth in utero and to reduce the hernial contents in CDH. The purpose of this study was to determine the effect of reversible fetal tracheal occlusion on tracheal pressure and lung development. Nine fetal sheep were divided into two groups. Group 1 had intratracheal balloons placed, and the balloons were left inflated for 21 to 28 days. Group 2 consisted of littermates that served as controls. They either had uninflated balloons placed or were left unoperated. Tracheal pressure measurements were recorded periodically, and the amniotic fluid pressure served as a reference. The animals were killed near term, and the lungs, heart, and liver were weighed and corrected for body weight. Standard morphometry was used to compare the lungs further, and the lung DNA and protein content were measured. Tracheal damage from the balloon catheter also was assessed. The tracheal pressure was 3.85 (+/- .49 SE) mm Hg in experimental animals, and it averaged -0.27 (+/- .27 SE) mm Hg in controls (P < .0001). Tracheal occlusion increased lung weight and volume by two to three times (P < .0001 and P < .0006, respectively) while heart and liver weights remained similar to those of controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
E Hashim; J M Laberge; M F Chen; E W Quillen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  30     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-12-15     Completed Date:  1995-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1172-7     Citation Subset:  IM    
Affiliation:
Department of Surgery, Montreal Children's Hospital, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / physiology
Animals
Balloon Dilatation / adverse effects,  instrumentation
Body Weight
DNA / analysis
Embryonic and Fetal Development
Exudates and Transudates
Female
Fetal Diseases / surgery
Fetal Organ Maturity
Heart / embryology
Hernia, Diaphragmatic / embryology,  surgery
Humans
Infant, Newborn
Ligation
Liver / embryology
Lung / chemistry,  embryology*
Organ Size
Persistent Fetal Circulation Syndrome / prevention & control
Pregnancy
Pressure
Proteins / analysis
Pulmonary Alveoli / embryology
Sheep
Trachea / embryology*,  injuries,  surgery*
Chemical
Reg. No./Substance:
0/Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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