Document Detail


Reversible structural alterations of undifferentiated and differentiated human neuroblastoma cells induced by phorbol ester.
MedLine Citation:
PMID:  1518842     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphological alterations in the structure of undifferentiated and morphologically differentiated human neuroblastoma cells induced by phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, were examined by video microscopy and immunomorphology. In undifferentiated cells, PMA induced the formation of motile actin-rich lamellas and of stable cylindrical processes rich in microtubules. Formation of stable processes resulted either from the collapse of lamellas or the movement of the cell body away from the base of a process. In differentiated cells, PMA induced the rapid extension of small lamellas and subsequent formation of short-lived elongated processes from the lateral edges of neurites. Additionally, growth cones exhibited enhanced modulation in shape after PMA treatment. These reversible reorganizations were similar to the actinoplast-tubuloplast transformations exhibited by PMA-treated fibroblasts. We suggest that actinoplast-tubuloplast reorganizations play essential roles in morphogenesis where stable cytoplasmic extensions are induced by external stimuli. In particular, PMA-induced reorganizations of neural cells in culture may be a model for morphological modulations that occur in nerve tissue.
Authors:
I S Tint; E M Bonder; H H Feder; C P Reboulleau; J M Vasiliev; I M Gelfand
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  89     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-10-07     Completed Date:  1992-10-07     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8160-4     Citation Subset:  IM    
Affiliation:
Laboratory of Mathematical Biology, Belozersky Institute for Physico-Chemical Biology, Moscow State University, Russia.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Cell Differentiation / drug effects*
Cytoskeleton / ultrastructure
Humans
Neurites / ultrastructure
Neuroblastoma / pathology*
Neurons / cytology
Protein Kinase C / physiology
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
HD-04467/HD/NICHD NIH HHS; HD-24649/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.7.11.13/Protein Kinase C
Comments/Corrections

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