| Reversible receptor methylation is essential for normal chemotaxis of Escherichia coli in gradients of aspartic acid. | |
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MedLine Citation:
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PMID: 2829179 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The chemotaxis of wild-type cells of Escherichia coli and double mutants lacking the methyltransferase and the methylesterase activities of the receptor modification system has been compared in spatial gradients of aspartic acid. Previous studies showing that a chemotactic response can be observed for the mutant raised questions about the role of methylation in the bacterial memory. To clarify the role of methylation, the redistribution of bacteria in stabilized defined gradients of aspartic acid was monitored by light scattering. There was no redistribution of the mutant cells in nonsaturating gradients of aspartic acid, but over the same range these mutant bacteria were observed to respond and to adapt during tethering experiments. In large saturating gradients of aspartate, slight movement of the mutant up the gradient was observed. These results show that dynamic receptor methylation is required for the chemotactic response to gentle gradients of aspartic acid and that methylation resets to zero and is part of the normal wild-type memory. There are certain gradients, however, in which the methylation-deficient mutants show chemotactic ability, thus explaining the apparent anomaly. |
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Authors:
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R M Weis; D E Koshland |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 85 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1988 Jan |
Date Detail:
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Created Date: 1988-03-02 Completed Date: 1988-03-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 83-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of California, Berkeley 94720. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid
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metabolism* Cell Movement Chemotaxis* Escherichia coli / enzymology, genetics, physiology* Kinetics Methylation Methyltransferases / metabolism* Mutation Receptors, Amino Acid* Receptors, Cell Surface / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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5 RO1 DK09765/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Amino Acid; 0/Receptors, Cell Surface; 0/aspartic acid receptor; 56-84-8/Aspartic Acid; EC 2.1.1.-/Methyltransferases; EC 2.1.1.-/chemotaxis methyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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