Document Detail


Reversible phenotypic modulation induced by deprivation of exogenous essential fatty acids.
MedLine Citation:
PMID:  3199798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Essential fatty acid deficiency, produced by deprivation of omega-6 and omega-3 fatty acids, is a condition characterized by renal disease, dermatitis, and infertility. Although many of the biochemical aspects of this disorder have been investigated, little is known about the ultrastructural changes induced by essential fatty acid deficiency. Using a unique fatty acid-deficient cell line (EFD-1), which demonstrates the in vivo fatty acid changes of essential fatty acid deficiency, and the prostaglandin E2-producing mouse fibrosarcoma line from which it was derived (HSDM1C1), we correlated ultrastructural and biochemical changes induced by prolonged deprivation of all exogenous lipids and subsequent repletion of selected essential fatty acids. We found that in cells deprived of all exogenous lipids, there was dilation of rough endoplasmic reticulum and an associated defect in protein secretion; these changes were specifically reversed by arachidonate. There was also an accumulation of secondary lysosomes containing degraded membranes in these cells with an associated increase in phospholipids relative to parent HSDM1C1 cells. Cytoplasmic lipid bodies present in parent cells disappeared, with an associated decrease in triacylglycerol. After just 2 days in lipid-free medium, all these changes were apparent, and prostaglandin E2 production was markedly impaired despite normal amounts of cellular arachidonate. Incubation of EFD-1 cells with arachidonate, the major prostaglandin precursor fatty acid, induced a reversion to the HSDM1C1 phenotype, whereas other fatty acids were totally ineffective. These results indicate changes in fatty acid metabolism in essential fatty acid deficiency are associated with marked alterations in ultrastructure and secretion of protein from cells.
Authors:
M Laposata; M Minda; A M Capriotti; E J Hartman; E E Furth; R V Iozzo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  59     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-01-26     Completed Date:  1989-01-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  838-47     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory of Medicine, University of Pennsylvania School of Medicine, Philadelphia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Endoplasmic Reticulum / ultrastructure*
Fatty Acids, Essential / deficiency*
Fibrosarcoma
Lipid Metabolism*
Lysosomes / ultrastructure*
Mice
Microbodies / ultrastructure*
Microscopy, Electron
Phenotype
Protein Biosynthesis
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
AG-05707/AG/NIA NIH HHS; CA-39481/CA/NCI NIH HHS; DK-37454/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Essential

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