Document Detail


Reversible microcarrier-mediated junctional communication between endothelial and smooth muscle cell monolayers: an in vitro model of vascular cell interactions.
MedLine Citation:
PMID:  4068671     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Junctional communication between cultured monolayers of aortic endothelial and smooth muscle cells was established as an in vitro model of vessel wall cell interactions. Confluent monolayers of endothelial cells on microcarriers placed on the surface of a smooth muscle cell monolayer became attached within 1 hour. After 3 hours of contact co-culture, approximately 4% of each monolayer was involved in heterocellular attachment (range 2% to 6%; 4 to 12 cells per microcarrier). Endothelial-smooth muscle cell junctions formed between the two cell populations at the sites of attachment on the lower surface of each microcarrier. When either endothelial cells or smooth muscle cells were prelabeled with [3H]uridine, intracellular nucleotide was rapidly transferred across the region of heterocellular attachment to the complementary cell population. Heterocellular gap junctional transfer was inhibited when endothelial-smooth muscle attachment was prevented by slowly rocking the culture vessel. No evidence of nucleotide transfer was obtained when endothelial cells were co-cultured with MDCK cells incapable of forming gap junctions. Contact co-culture of endothelium and smooth muscle cells were reversed by gentle agitation of the cultures; the microcarriers detached allowing the recovery of pure cell populations. The contact co-culture technique is well suited to studies of vascular cell interactions and is of general applicability to anchorage dependent cells.
Authors:
P F Davies; P Ganz; P S Diehl
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  53     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-16     Completed Date:  1986-01-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  710-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cells, Cultured
Endothelium / cytology*,  ultrastructure
Intercellular Junctions / ultrastructure*
Microscopy, Electron, Scanning
Models, Biological
Muscle, Smooth, Vascular / cytology*,  ultrastructure
Grant Support
ID/Acronym/Agency:
HL01045/HL/NHLBI NIH HHS; HL22602/HL/NHLBI NIH HHS; HL24612/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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