Document Detail

Reversible inhibition of cellular respiration by nitric oxide in vascular inflammation.
MedLine Citation:
PMID:  11709390     Owner:  NLM     Status:  MEDLINE    
Incubation of rat aortas with endotoxin and interferon-gamma for 24 h resulted in an aortic oxygen consumption that was substantially inhibited and strongly oxygen dependent (37% inhibition at 160 microM O(2) and 62% inhibition at 80 microM O(2) relative to untreated aortas). This respiratory inhibition was reversed by a nitric oxide (NO) scavenger (oxyhemoglobin) or by an inhibitor of inducible NO synthase [N-(3-(aminomethyl)benzyl)acetamide x 2HCl, 1400W], but not by an inhibitor of soluble guanylate cyclase (1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one). Addition of 1 microM NO to untreated aortas caused rapid and reversible inhibition of oxygen consumption that was greater at lower oxygen concentrations. Incubation of endothelial cells isolated from rat aortas with endotoxin and interferon-gamma for 24 h resulted in a steady-state NO concentration of approximately 0.5 microM and 90% inhibition of cellular oxygen consumption that was immediately reversed by an NO scavenger (oxyhemoglobin). These results suggest that during inflammation and sepsis, tissue respiration may be substantially reduced due to inhibition by NO of cytochrome oxidase.
V Borutaite; A Matthias; H Harris; S Moncada; G C Brown
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  281     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-15     Completed Date:  2002-01-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2256-60     Citation Subset:  IM    
Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom.
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MeSH Terms
Amidines / pharmacology
Antineoplastic Agents / pharmacology
Aorta, Thoracic / cytology,  metabolism*
Benzylamines / pharmacology
Cell Respiration / physiology
Cells, Cultured
Endothelium, Vascular / cytology,  enzymology
Enzyme Activation / drug effects,  physiology
Enzyme Inhibitors / pharmacology
Interferon-gamma / pharmacology
Lipopolysaccharides / pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Synthase / antagonists & inhibitors,  metabolism
Nitric Oxide Synthase Type II
Oxadiazoles / pharmacology
Oxyhemoglobins / pharmacology
Quinoxalines / pharmacology
Rats, Wistar
Vasculitis / metabolism*
Reg. No./Substance:
0/1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; 0/Amidines; 0/Antineoplastic Agents; 0/Benzylamines; 0/Enzyme Inhibitors; 0/Lipopolysaccharides; 0/N-(3-(aminomethyl)benzyl)acetamidine; 0/Oxadiazoles; 0/Oxyhemoglobins; 0/Quinoxalines; 10102-43-9/Nitric Oxide; 82115-62-6/Interferon-gamma; EC Oxide Synthase; EC Oxide Synthase Type II; EC protein, rat

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