| Reversible independent alterations in glucose transport and metabolism in cultured human cells deprived of glucose. | |
| | |
MedLine Citation:
|
PMID: 956278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have measured uptake of 3H-hexoses into diploid human cells by exposing them to brief pulses of isotopic sugar during the log-growth, subconfluent-growth, and confluent-growth (contact inhibited) phases of the strain HSWP derived from human skin. 3H-deoxyglucose appears to be taken up three times faster than 3H-glucose. After exposure to 3H-glucose for longer than one minute, the cells excrete approximately 70% of the isotope into the medium as lactate. If lactate production (and hence excretion) is abolished by treating the cellls with iodoacetic acid or dinitrofluorobenzene, neither of which inhibits transport, the uptake of 3H-glucose is found to be in fact somewhat larger than that of 3H-deoxyglucose. If cells are deprived of glucose for 24 hours, apparent uptake of 3H-glucose is enhanced 10-fold or more. This latter increase is accounted for by 2- to 3-fold enhancement of true transport plus retention of greater than 90% of the radioactivity, since little lactate is formed or excreted in glucose-deprived cells. Deoxyglucose, galactose, or pyruvate when present during glucose deprivation each have quantitatively different effects on the cells' capacity to produce lactate from a short pulse of glucose, but none of them prevents the enhancement of hexose transport. After restoration of 5 mM glucose to starved cells, their metabolsim returns to normal (in the sense that approximately 70% of the glucose taken up in a pulse is again excreted as lactate), with a half-time of 0.5 hour; but the transport of hexoses returns to control levels much more slowly, with a half-time of approximately 6 hours. The two processes appear to be independently regulated. |
| | |
Authors:
|
D W Salter; J S Cook |
Related Documents
:
|
1640848 - Vitamin e restores reduced prostacyclin synthesis in aortic endothelial cells cultured ... 12432458 - Factors affecting the antibacterial activity of the ruminal bacterium, streptococcus bo... 20722628 - Glucose deficiency reduces collagen synthesis in breast cancer mcf7 cells. 3218078 - Treponema hyodysenteriae growth under various culture conditions. 7769098 - Interleukin-1 beta induces cardiac myocyte growth but inhibits cardiac fibroblast proli... 22365358 - Characterization of the "viable but nonculturable" (vbnc) state in the wine spoilage ye... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 89 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1976 Sep |
Date Detail:
|
Created Date: 1976-11-01 Completed Date: 1976-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 143-55 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport, Active
/
drug effects Cell Line Deoxyglucose / metabolism Dinitrofluorobenzene / pharmacology Galactose / metabolism Glucose / metabolism* Iodoacetates / pharmacology Kinetics Lactates / metabolism Methylglucosides / metabolism Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
|
0/Iodoacetates; 0/Lactates; 0/Methylglucosides; 154-17-6/Deoxyglucose; 26566-61-0/Galactose; 50-99-7/Glucose; 70-34-8/Dinitrofluorobenzene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cell cycle dependent changes in potassium transport.
Next Document: Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA.