Document Detail

Reversible contraception with chloroform extract of Carica papaya Linn. seeds in male rabbits.
MedLine Citation:
PMID:  10080301     Owner:  NLM     Status:  MEDLINE    
The contraceptive efficacy and reversibility of the chloroform extract of the seeds of Carica papaya in adult male rabbits were investigated. Eighteen adult male rabbits were divided into three groups of six animals each; Group I--control, Group II--administered chloroform extract of the seeds of Carica papaya at 20 mg/animal/d for 150 d by gavage, and Group III--administered the seed extract at 50 mg/animal/d for 150 d. Body weight and organ weight, semen analysis, sperm morphology by scanning electron microscopy, semen biochemistry, histology of the testis, haematology, serum clinical biochemistry, and the fertility status of the control and the treated animals were evaluated. Body weight and the weight of the testis, epididymis, seminal vesicles, and prostate did not show appreciable changes. Sperm concentration showed a gradual decline, reached severe oligospermia (fewer than 20 million/mL) after 75 d treatment, and attained uniform azoospermia after 120 d treatment. Sperm motility and viability were severely affected after 45 d treatment and reached less than 1% after 75 d treatment. The morphology of the spermatozoa by scanning electron microscopy revealed membrane damage in the acrosome, bent midpiece, coiled tail, and detached head and tail. The levels of fructose, glycerylphosphorylcholine, acid phosphatase, and lactate dehydrogenase in the seminal plasma were unaltered. Histology of the testis revealed arrest of spermatogenesis beyond the level of spermatocytes. No toxicity was evident from the haematology and serum biochemistry parameters. The libido of the treated animals was unaffected and the fertility rate was zero. The effects were comparable in both the dose regimens (Groups II and III) and were restored to normal 45 d after withdrawal of the treatment.
N K Lohiya; N Pathak; P K Mishra; B Manivannan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive toxicology (Elmsford, N.Y.)     Volume:  13     ISSN:  0890-6238     ISO Abbreviation:  Reprod. Toxicol.     Publication Date:    1999 Jan-Feb
Date Detail:
Created Date:  1999-05-11     Completed Date:  1999-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803591     Medline TA:  Reprod Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
Department of Zoology, University of Rajasthan, Jaipur, India.
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MeSH Terms
Blood Cell Count / drug effects
Blood Chemical Analysis
Body Weight / drug effects
Chloroform / chemistry
Contraceptive Agents, Male / pharmacology*,  toxicity
Fertility / drug effects
Fruit / chemistry*
Libido / drug effects
Organ Size / drug effects
Plant Extracts / pharmacology*,  toxicity
Seeds / chemistry
Semen / drug effects
Testis / anatomy & histology,  cytology,  drug effects
Reg. No./Substance:
0/Contraceptive Agents, Male; 0/Plant Extracts; 67-66-3/Chloroform

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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