Document Detail

Reversible photoswitching of spiropyran-conjugated semiconducting polymer dots.
MedLine Citation:
PMID:  23033991     Owner:  NLM     Status:  MEDLINE    
Semiconducting polymer dots (Pdots) recently have emerged as a new class of ultrabright fluorescent probes with promising applications in biological detection and imaging. We developed photoswitchable Pdots by conjugating photochromic spiropyran molecules onto poly[9,9-dioctylfluorenyl-2,7-diyl)-co-1,4-benzo-{2,1'-3}-thiadiazole)] (PFBT). The modulation of fluorescence was achieved by ultraviolet irradiation, which converted spiropyran into its visible-absorbing merocyanine form. The merocyanine efficiently quenched the fluorescence of PFBT via Förster resonance energy transfer (FRET). We then reversed the quenching by subsequent irradiation with visible light to get back the fluorescence of PFBT. This FRET-based photomodulation of Pdot fluorescence could be repeated multiple times. We next conjugated biomolecules onto the surface of these photoswitchable Pdots and demonstrated their specific cellular and subcellular labeling to different types of cells without any noticeable nonspecific binding. We anticipate these photoswitchable and biocompatible Pdots will be useful in developing bioimaging techniques in the future.
Yang-Hsiang Chan; Maria Elena Gallina; Xuanjun Zhang; I-Che Wu; Yuhui Jin; Wei Sun; Daniel T Chiu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-22
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-06     Completed Date:  2013-11-26     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9431-8     Citation Subset:  IM    
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MeSH Terms
Benzopyrans / chemistry*
Fluorenes / chemistry
HeLa Cells
Indoles / chemistry*
MCF-7 Cells
Molecular Imaging
Nitro Compounds / chemistry*
Polymers / chemistry*
Spectrometry, Fluorescence
Streptavidin / chemistry
Grant Support
Reg. No./Substance:
0/Benzopyrans; 0/Fluorenes; 0/Indoles; 0/Nitro Compounds; 0/Polymers; 0/poly(9,9'-bis(6''-N,N,N-trimethylammonium)hexyl)fluorene-co-alt-4,7-(2,1,3-benzothiadiazole) dibromide; 0/spiropyran; 9013-20-1/Streptavidin

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