Document Detail


Reversibility of tributyltin-chloride-induced protein synthesis inhibition after ATP recovery in HEL-30 cells.
MedLine Citation:
PMID:  2389259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of tributyltin chloride (TBT) on ATP levels and protein synthesis was investigated in a murine epidermal cell line (HEL-30). Five minutes after exposure to 10(-5) M TBT, cellular levels of ATP decreased by 57% and protein synthesis was significantly inhibited. The effect of ATP was stable up to 2 h incubation, whereas inhibition of protein synthesis increased with time of treatment. Partial (1 h) or complete (2 h) recovery of the two cell functions was observed when dithiothreitol (DTT) was added to the medium, 5 min after the damage and in the absence of TBT. The sequence of the observed effects suggests that TBT inhibition of protein synthesis is due to impairment of ATP production.
Authors:
M Marinovich; B Viviani; C L Galli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology letters     Volume:  52     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  311-7     Citation Subset:  IM    
Affiliation:
Institute of Pharmacological Sciences, University of Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / biosynthesis*
Animals
Cell Line / drug effects
Dithiothreitol / pharmacology
Leucine / metabolism
Mice
Protein Biosynthesis*
Trialkyltin Compounds / antagonists & inhibitors,  toxicity*
Chemical
Reg. No./Substance:
0/Trialkyltin Compounds; 3483-12-3/Dithiothreitol; 56-65-5/Adenosine Triphosphate; 61-90-5/Leucine; 688-73-3/tributyltin

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