| Reversibility of granulocyte adhesion using polyamine-grafted nylon-6 as a new column substrate for granulocyte separation. | |
| | |
MedLine Citation:
|
PMID: 4084643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Reversibility of leucocytes adhered onto the surface of polyamine-grafted nylon-6 was investigated to estimate its feasibility as a new column substrate for granulocyte separation from whole blood. Polyamine-grafted nylon-6 was synthesized by a radical polymerization of 2-diethylaminoethyl methacrylate monomer onto nylon-6. The surfaces of these graft copolymers were found to show a microphase-separated structure composed of island-like phases of cationic polyamine and continuous phases of nonionic nylon-6. Interaction between leucocytes and these copolymer surfaces was studied by passing rabbit heparinized blood through a column packed with glass beads precoated with these copolymers. Columns of these copolymers showed a selective adhesion of granulocytes among leucocyte populations. Also, the adhering granulocytes were able to be recovered from the column by a gentle elution procedure. From these results, it was concluded that polyamine-grafted nylon-6 having a microphase-separated structure is suitable for use as a column substrate in granulocyte separation from whole blood. |
| | |
Authors:
|
N Yui; K Sanui; N Ogata; K Kataoka; T Okano; Y Sakurai |
Related Documents
:
|
7847973 - Effect of topical vasodilators on internal mammary arteries. 9527283 - Migration resistant, blood-compatible plasticized polyvinyl chloride for medical and re... 6117193 - The effects of pindolol and prazosin on hand blood flow in patients with cold extremiti... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biomaterials Volume: 6 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 1985 Nov |
Date Detail:
|
Created Date: 1986-03-07 Completed Date: 1986-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 409-15 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Azepines* Caprolactam* / analogs & derivatives Cell Adhesion Cell Separation / methods* Cells, Cultured Granulocytes / cytology* Male Polymers* Rabbits Surface Properties |
| Chemical | |
Reg. No./Substance:
|
0/Azepines; 0/Polymers; 105-60-2/Caprolactam; 25038-54-4/nylon 6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities.
Next Document: Changes in protein adsorption on polycarbonate due to L-ascorbic acid.