| Reversibility and binding kinetics of Thermobifida fusca cellulases studied through fluorescence recovery after photobleaching microscopy. | |
| | |
MedLine Citation:
|
PMID: 21396764 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Cellulases are enzymes capable of depolymerizing cellulose. Understanding their interactions with cellulose can improve biomass saccharification and enzyme recycling in biofuel production. This paper presents a study on binding and binding reversibility of Thermobifida fusca cellulases Cel5A, Cel6B, and Cel9A bound onto Bacterial Microcrystalline Cellulose. Cellulase binding was assessed through fluorescence recovery after photobleaching (FRAP) at 23, 34, and 45°C. It was found that cellulase binding is only partially reversible. For processive cellulases Cel6B and Cel9A, an increase in temperature resulted in a decrease of the fraction of cellulases reversibly bound, while for endocellulase Cel5A this fraction remained constant. Kinetic parameters were obtained by fitting the FRAP curves to a binding-dominated model. The unbinding rate constants obtained for all temperatures were highest for Cel5A and lowest for Cel9A. The results presented demonstrate the usefulness of FRAP to access the fast binding kinetics characteristic of cellulases operating at their optimal temperature. |
| | |
Authors:
|
Jose M Moran-Mirabal; Jacob C Bolewski; Larry P Walker |
Related Documents
:
|
21431564 - Prediction of chemical-protein binding activity using contrast graph patterns. 21496214 - Entropic fragment based approach to aptamer design. 21210044 - Titanocene anticancer complexes and their binding mode of action to human serum albumin... 8805524 - Crystal structures of murine polyomavirus in complex with straight-chain and branched-c... 1964224 - Characterization of [3h]alprazolam binding to central benzodiazepine receptors. 2848184 - Determination and characterization of a cannabinoid receptor in rat brain. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-2-19 |
Journal Detail:
|
Title: Biophysical chemistry Volume: - ISSN: 1873-4200 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
|
Created Date: 2011-3-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403171 Medline TA: Biophys Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier B.V. All rights reserved. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Suctioning via the tube during endotracheal intubation in a model of severe upper airway haemorrhage...
Next Document: The circular dichroism and differential scanning calorimetry study of the properties of DNA aptamer ...