Document Detail

Reversed unfolding-refolding process of cobra neurotoxin.
MedLine Citation:
PMID:  7326234     Owner:  NLM     Status:  MEDLINE    
Circular dichroism and nuclear magnetic resonance spectroscopies have been used to study the unfolding process of cobrotoxin upon addition of fluoro alcohols/or sodium dodecyl sulfate to its aqueous solution. In each final unfolded state, the protein had its disulfide bonds intact. The unfolding process has been found to be reversible in the case of fluoro alcohol/water mixtures, while no such reversibility was found in the case of sodium dodecyl sulfate. However, when hexafluoro-2-propanol is added to the sodium dodecyl sulfate unfolded protein, refolding is induced. The mechanism of unfolding is discussed in terms of the different interactions which govern the protein conformation in solution.
A Gałat; J P Degelaen; C C Yang; E R Blout
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  20     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-04-22     Completed Date:  1982-04-22     Revised Date:  2011-12-06    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7415-23     Citation Subset:  IM    
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MeSH Terms
Chemical Phenomena
Circular Dichroism
Cobra Neurotoxin Proteins / analysis*
Cobra Venoms / analysis*
Magnetic Resonance Spectroscopy
Protein Conformation
Proteins / analysis*
Sodium Dodecyl Sulfate
Grant Support
Reg. No./Substance:
0/Cobra Neurotoxin Proteins; 0/Cobra Venoms; 0/Proteins; 151-21-3/Sodium Dodecyl Sulfate

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