Document Detail


Reversed unfolding-refolding process of cobra neurotoxin.
MedLine Citation:
PMID:  7326234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Circular dichroism and nuclear magnetic resonance spectroscopies have been used to study the unfolding process of cobrotoxin upon addition of fluoro alcohols/or sodium dodecyl sulfate to its aqueous solution. In each final unfolded state, the protein had its disulfide bonds intact. The unfolding process has been found to be reversible in the case of fluoro alcohol/water mixtures, while no such reversibility was found in the case of sodium dodecyl sulfate. However, when hexafluoro-2-propanol is added to the sodium dodecyl sulfate unfolded protein, refolding is induced. The mechanism of unfolding is discussed in terms of the different interactions which govern the protein conformation in solution.
Authors:
A Gałat; J P Degelaen; C C Yang; E R Blout
Related Documents :
19665594 - Biophysical investigations on the aggregation and thermal unfolding of harpin(pss) and ...
15491624 - Probing the high energy states in proteins by proteolysis.
8332604 - Structural energetics of the molten globule state.
1537844 - Folding mechanism of mutant human lysozyme c77/95a with increased secretion efficiency ...
16786224 - Soluble proteins in insect chemical communication.
20055754 - Enhanced hepatic nrf2 activation after ursodeoxycholic acid treatment in patients with ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  20     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-04-22     Completed Date:  1982-04-22     Revised Date:  2011-12-06    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7415-23     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Circular Dichroism
Cobra Neurotoxin Proteins / analysis*
Cobra Venoms / analysis*
Magnetic Resonance Spectroscopy
Protein Conformation
Proteins / analysis*
Sodium Dodecyl Sulfate
Grant Support
ID/Acronym/Agency:
AM07300/AM/NIADDK NIH HHS; PR00995/PR/OCPHP CDC HHS
Chemical
Reg. No./Substance:
0/Cobra Neurotoxin Proteins; 0/Cobra Venoms; 0/Proteins; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Measurement of free magnesium in perfused and ischemic arrested heart muscle. A quantitative phospho...
Next Document:  Purification and characterization of avian dopamine beta-hydroxylase.