Document Detail


Reversed-phase chromatography of novel biologically effective aminoacridine-N-glycosides by OPLC.
MedLine Citation:
PMID:  2745663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reversed-phase chromatographic behavior of novel biologically active aminoacridine-N-glycosides is studied. The chromatographic experiments are performed with overpressurized layer chromatography. Weak ion pairs are formed with methanesulfonic acid, but only at low concentrations of the ion-pairing reagent. The retention seems to involve a reversed-phase mechanism. The base compounds only slightly modify the retention, while the number and polarity of the substituents have larger effects. The pH dependence of the retention is very typical for the aminoacridine-N-glycosides, and it plays an important role in the separation. The monoglycosides are completely separated from the corresponding base compounds, as are the diglycosides from the monoglycosides, on RP-2, RP-8, and RP-18 layers with eluents containing 30 to 60% acetonitrile and at least 0.005 M ammonium carbonate at pH 4 to 6.
Authors:
K Kovács-Hadady; A Kovács
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  27     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-08-25     Completed Date:  1989-08-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-4     Citation Subset:  IM    
Affiliation:
BIOGAL Pharmaceutical Works, Debrecen, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Acridines / analysis*
Acriflavine / analogs & derivatives,  analysis*
Aminoacridines / analysis*
Chromatography, High Pressure Liquid / methods*
Glycosides / analysis*
Hydrogen-Ion Concentration
Proflavine / analogs & derivatives,  analysis*
Chemical
Reg. No./Substance:
0/Acridines; 0/Aminoacridines; 0/Glycosides; 8048-52-0/Acriflavine; 92-62-6/Proflavine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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