Document Detail


Reversed-phase chromatography of interleukin-2 muteins.
MedLine Citation:
PMID:  3488324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human recombinant interleukin-2 (IL-2) and related species have been characterized by chemical modifications, tryptic digestion, and cyanogen bromide digestion. The oxidation states of the cysteines and methionines in several IL-2 muteins have been determined. Reversed-phase high-performance liquid chromatography allowed us to distinguish the modifications in these muteins and to correlate retention behavior with their structure.
Authors:
M Kunitani; P Hirtzer; D Johnson; R Halenbeck; A Boosman; K Koths
Related Documents :
24616054 - Simultaneous determination of cotinine and trans-3-hydroxycotinine in urine by automate...
23590404 - Orbitrap mass spectrometry.
19427484 - A chemometric study of chromatograms of tea extracts by correlation optimization warpin...
24847944 - Detection of yersinia pestis in environmental and food samples by intact cell immunocap...
11902964 - Identification of anthocyanin-flavanol pigments in red wines by nmr and mass spectrometry.
19703704 - A pilot simulation study of arsenic tracked from cca-treated decks onto carpets.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  359     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-09-16     Completed Date:  1986-09-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  391-402     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cyanogen Bromide
Cysteine / analysis
Humans
Hydrolysis
Interleukin-2 / analysis*
Methionine / analysis
Oxidation-Reduction
Recombinant Proteins / analysis
Trypsin
Chemical
Reg. No./Substance:
0/Interleukin-2; 0/Recombinant Proteins; AE28F7PNPL/Methionine; EC 3.4.21.4/Trypsin; K848JZ4886/Cysteine; OS382OHJ8P/Cyanogen Bromide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tomographic mapping of kinetic rate constants in the fluorodeoxyglucose model using dynamic positron...
Next Document:  Regulation of the activities of 17-hydroxylase and 17,20-desmolase in the human adrenal cortex: kine...