Document Detail

Reversed-phase chromatography of interleukin-2 muteins.
MedLine Citation:
PMID:  3488324     Owner:  NLM     Status:  MEDLINE    
Human recombinant interleukin-2 (IL-2) and related species have been characterized by chemical modifications, tryptic digestion, and cyanogen bromide digestion. The oxidation states of the cysteines and methionines in several IL-2 muteins have been determined. Reversed-phase high-performance liquid chromatography allowed us to distinguish the modifications in these muteins and to correlate retention behavior with their structure.
M Kunitani; P Hirtzer; D Johnson; R Halenbeck; A Boosman; K Koths
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  359     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-09-16     Completed Date:  1986-09-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  391-402     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cyanogen Bromide
Cysteine / analysis
Interleukin-2 / analysis*
Methionine / analysis
Recombinant Proteins / analysis
Reg. No./Substance:
0/Interleukin-2; 0/Recombinant Proteins; AE28F7PNPL/Methionine; EC; K848JZ4886/Cysteine; OS382OHJ8P/Cyanogen Bromide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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