| Reversed-phase chromatography of interleukin-2 muteins. | |
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MedLine Citation:
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PMID: 3488324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human recombinant interleukin-2 (IL-2) and related species have been characterized by chemical modifications, tryptic digestion, and cyanogen bromide digestion. The oxidation states of the cysteines and methionines in several IL-2 muteins have been determined. Reversed-phase high-performance liquid chromatography allowed us to distinguish the modifications in these muteins and to correlate retention behavior with their structure. |
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Authors:
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M Kunitani; P Hirtzer; D Johnson; R Halenbeck; A Boosman; K Koths |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 359 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-09-16 Completed Date: 1986-09-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 391-402 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cyanogen Bromide Cysteine / analysis Humans Hydrolysis Interleukin-2 / analysis* Methionine / analysis Oxidation-Reduction Recombinant Proteins / analysis Trypsin |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-2; 0/Recombinant Proteins; 506-68-3/Cyanogen Bromide; 52-90-4/Cysteine; 63-68-3/Methionine; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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