Document Detail


Reverse transcriptase-polymerase chain reaction for prostate-specific antigen in the molecular staging of pelvic surgical margins after radical prostatectomy.
MedLine Citation:
PMID:  11337316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To examine the application of reverse transcriptase-polymerase chain reaction (RT-PCR) to assist in prostate-specific antigen (PSA) detection in the surgical margins after radical prostatectomy (RP). The risk of local recurrence increases considerably in the presence of extracapsular tumor growth and/or positive surgical margins at RP. Although this makes it possible to identify patients with an increased risk of local recurrence, precise predictions cannot be made. A more precise assessment is desirable mainly for early planning of adjuvant therapy. METHODS: Ninety-five patients with clinically organ-confined prostate cancer (CaP) underwent RP. After removing the gland, biopsies were obtained from four defined areas of the prostatic fossa and processed for RT-PCR for PSA detection. Sixteen patients with muscle-invasive bladder carcinoma who underwent radical cystoprostatectomy served as controls. RESULTS: Thirty-two of 95 patients with CaP (35%) had at least one positive molecular margin indicating an expression for PSA; 19 of 48 (39%) of these had an organ-confined tumor stage according to conventional histology and 13 of 47 (28%) had tumor growth beyond the prostate. A statistically significant correlation between the frequency of positive molecular margins and clinical data was only observed in the group with disease greater than Stage pT2. All controls had negative molecular margins (P = 0.012). CONCLUSIONS: On the basis of the results obtained, molecular diagnostic RT-PCR for PSA detection in the surgical margins after RP seems to be an interesting supplementary tool for monitoring the course and establishing the prognosis. Long-term follow-up of these patients is needed to demonstrate the clinical value of molecular diagnostics of surgical margins during RP.
Authors:
B Straub; M Müller; H Krause; C Goessl; M Schrader; R Heicappell; K Miller
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Urology     Volume:  57     ISSN:  1527-9995     ISO Abbreviation:  Urology     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-04     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0366151     Medline TA:  Urology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1006-11     Citation Subset:  IM    
Affiliation:
Department of Urology, Klinikum Benjamin Franklin, Freie Universität Berlin, Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / chemistry*,  pathology,  surgery*
Aged
Humans
Male
Middle Aged
Neoplasm Staging
Prostate / chemistry*,  pathology
Prostate-Specific Antigen / analysis*
Prostatectomy*
Prostatic Neoplasms / chemistry*,  pathology,  surgery*
Reverse Transcriptase Polymerase Chain Reaction / statistics & numerical data*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
EC 3.4.21.77/Prostate-Specific Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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