| Reverse transcriptase in situ polymerase chain reaction in atypical mycobacterial adenitis. | |
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MedLine Citation:
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PMID: 8906057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis. DESIGN: Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis. SETTING: University medical center caring for both ambulatory and hospitalized children. PATIENTS: Twelve pediatric patients (all younger than 9 years) with cervicofacial masses. INTERVENTION: Surgical excision of the presenting mass. MAIN OUTCOME MEASURES: Reverse transcriptase in situ PCR was used to detect mycobacterial RNA in excised tissue. All specimens were also cultured and stained for acid-fast bacilli. RESULTS: Reverse transcriptase in situ PCR was positive for NTM in 7 of 12 cases. CONCLUSIONS: Infection with NTM may be an extremely indolent process, and the success of RT in situ PCR depends on the presence of mycobacterial nucleic acids. Even in cases in which the findings of RT in situ PCR were positive, infected cells were few in number. Because of the sparsity of infection in the positive cases, NTM may be even more rare in the negative cases, ie, those in which mycobacterial nucleic acids do not exist and cannot be detected by any means, including RT in situ PCR. Although RT in situ PCR, cultures, stains for acid-fast bacilli, and tuberculin tests using purified protein derivative are all helpful in diagnosing NTM cervical adenitis, when nucleic acids are present RT in situ PCR is the simplest, most reliable, and quickest to perform and the results are easiest to interpret. |
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Authors:
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M M April; J M Garelick; G J Nuovo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of otolaryngology--head & neck surgery Volume: 122 ISSN: 0886-4470 ISO Abbreviation: Arch. Otolaryngol. Head Neck Surg. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1996-12-04 Completed Date: 1996-12-04 Revised Date: 2006-03-28 |
Medline Journal Info:
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Nlm Unique ID: 8603209 Medline TA: Arch Otolaryngol Head Neck Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1214-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University Hospital and Medical Center, State University of New York at Stony Brook, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Child Child, Preschool Humans Infant Lymphadenitis / diagnosis* Mycobacterium Infections, Atypical / diagnosis* Neck Polymerase Chain Reaction* Retrospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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