Document Detail


Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria.
MedLine Citation:
PMID:  19419007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reverse transcriptase (RT) activity has been reported in bivalves affected by haemic neoplasia (HN). Since all retroviruses have RT, detection of RT activity was regarded as evidence for the retroviral etiology of HN. This study investigates the relationship between RT levels and the progress of HN as indicated by percentages of tetraploid cells in soft-shell clams Mya arenaria. The percentages of tetraploid cells were estimated by flow cytometry, and the RT levels were quantified using TaqMan product-enhanced RT (TM-PERT) assay. Results demonstrated that the amount of RT was positively correlated with the percentage of tetraploid cells circulating in clam haemolymph (R2 = 0.974, p < 0.001). Compared to HN-negative clams (<5% tetraploid cells), 2 stages with significantly elevated levels of RT activity were observed: the first stage at approximately 10 to approximately 20% tetraploid cells, and the second at approximately 30 to approximately 80% tetraploid cells (p < 0.01). These data support the well established fact from mammalian models that transformed cells express high levels of non-telomeric RT. The observed increase in RT levels at approximately 30% tetraploidy coincides with previously reported p53 gene expression. Taken together, this could indicate that using RT levels as an indicator of HN, > or = 30% tetraploidy is the stage at which the disease process undergoes a change, and perhaps becomes irreversible.
Authors:
M AboElkhair; A Siah; K F Clark; P McKenna; J Pariseau; S J Greenwood; F C J Berthe; A Cepica
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  84     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-05-07     Completed Date:  2009-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  57-63     Citation Subset:  IM    
Affiliation:
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island C1A 4P3, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bivalvia / virology*
Gene Expression Regulation, Viral / physiology
Hematologic Diseases / veterinary*,  virology
Neoplasms / enzymology*
RNA-Directed DNA Polymerase / metabolism*
Retroviridae / classification,  metabolism*
Chemical
Reg. No./Substance:
EC 2.7.7.49/RNA-Directed DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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