Document Detail


Reverse reaction of malic enzyme for HCO3- fixation into pyruvic acid to synthesize L-malic acid with enzymatic coenzyme regeneration.
MedLine Citation:
PMID:  18460807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Malic enzyme [L-malate: NAD(P)(+) oxidoreductase (EC 1.1.1.39)] catalyzes the oxidative decarboxylation of L-malic acid to produce pyruvic acid using the oxidized form of NAD(P) (NAD(P)(+)). We used a reverse reaction of the malic enzyme of Pseudomonas diminuta IFO 13182 for HCO(3)(-) fixation into pyruvic acid to produce L-malic acid with coenzyme (NADH) generation. Glucose-6-phosphate dehydrogenase (EC1.1.1.49) of Leuconostoc mesenteroides was suitable for coenzyme regeneration. Optimum conditions for the carboxylation of pyruvic acid were examined, including pyruvic acid, NAD(+), and both malic enzyme and glucose-6-phosphate dehydrogenase concentrations. Under optimal conditions, the ratio of HCO(3)(-) and pyruvic acid to malic acid was about 38% after 24 h of incubation at 30 degrees C, and the concentration of the accumulated L-malic acid in the reaction mixture was 38 mM. The malic enzyme reverse reaction was also carried out by the conjugated redox enzyme reaction with water-soluble polymer-bound NAD(+).
Authors:
Yoko Ohno; Toshihiko Nakamori; Haitao Zheng; Shin-ichiro Suye
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  72     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1278-82     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alginates / chemistry,  metabolism
Carbon Dioxide / metabolism*
Glucosephosphate Dehydrogenase / chemistry,  metabolism
Glucuronic Acid / chemistry,  metabolism
Hexuronic Acids / chemistry,  metabolism
Malate Dehydrogenase / chemistry,  metabolism*
Malates / metabolism*
NAD / metabolism*
Oxidation-Reduction
Pseudomonas / enzymology*
Pyruvic Acid / metabolism*
Chemical
Reg. No./Substance:
0/Alginates; 0/Hexuronic Acids; 0/Malates; 124-38-9/Carbon Dioxide; 127-17-3/Pyruvic Acid; 53-84-9/NAD; 576-37-4/Glucuronic Acid; 6915-15-7/malic acid; 9005-32-7/alginic acid; EC 1.1.1.37/Malate Dehydrogenase; EC 1.1.1.40/malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); EC 1.1.1.49/Glucosephosphate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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