Document Detail

Reverse correlation of E-cadherin and snail expression in oral squamous cell carcinoma cells in vitro.
MedLine Citation:
PMID:  11120485     Owner:  NLM     Status:  MEDLINE    
The loss of E-cadherin expression has been shown to correlate to the invasion and metastasis of many types of carcinomas. We established E-cadherin positive (HOC719-PE) and negative (HOC719-NE) clones from an oral squamous cell carcinoma (SCC). HOC719-PE cells showed epithelial morphology with E-cadherin expression in the cell membrane, whereas HOC719-NE cells demonstrated fibroblastic morphology without E-cadherin expression. In invasion assay and three dimensional culture, HOC719-NE showed much higher invasive ability than HOC719-PE cells. These cells expressed similar levels of mRNAs for alpha- and beta-catenin. However, HOC719-NE cells, but not HOC719-PE cells, showed strong expression of snail, a transcription factor implicated in the differentiation of epithelial cells into mesenchymal phenotype. This reverse expression of snail and E-cadherin was further observed in other SCC cells including HOC313, and TSU cells that we previously reported to show no expression of E-cadherin protein. These results indicated that the expression of snail has a key role for the acquisition of more invasive and metastatic phenotypes of SCC and the clones we reported here will be useful tools for understanding the mechanism of the transition from epithelial to mesenchymal SCC cells.
K Yokoyama; N Kamata; E Hayashi; T Hoteiya; N Ueda; R Fujimoto; M Nagayama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral oncology     Volume:  37     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  65-71     Citation Subset:  IM    
First Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Tokushima, Tokushima, Japan.
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MeSH Terms
Cadherins / genetics,  metabolism*
Carcinoma, Squamous Cell / metabolism*,  pathology
Cell Division
DNA-Binding Proteins / genetics,  metabolism*
Gene Expression
Mouth Neoplasms / metabolism*,  pathology
Neoplasm Invasiveness
Neoplasm Proteins / genetics,  metabolism*
RNA, Messenger / genetics
RNA, Neoplasm / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / genetics,  metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/Cadherins; 0/DNA-Binding Proteins; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Transcription Factors; 0/snail family transcription factors

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