Document Detail


Reversal of the vasoconstrictor step of hyperacute xenogeneic rejection of the liver by endothelin antagonists.
MedLine Citation:
PMID:  10807679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of endothelin in the initial vasoconstrictor step of hyperacute xenogeneic rejection was investigated. Isolated rat livers were perfused in recirculation. Perfusion with human sera provided an ex vivo model of hyperacute rejection in a discordant combination. Perfusion of 10% xenogeneic serum induced a marked (70%) and sustained reduction of the liver flow and induced the release of endothelin into the perfusion medium. In contrast, perfusion of 10% allogeneic serum or of 10% decomplemented human serum induced a weak (25%) and transient reduction of the liver flow and induced the release of minimal amounts of endothelin. The simultaneous administration of BQ 123 and BQ 788, the respective antagonists of ET(A) and ET(B) endothelin receptors, or that of bosentan, a mixed ET(A)/ET(B) antagonist, antagonized the vasoconstrictor effect of 10% xenogeneic human serum, as well as that of 10(-9) M endothelin-1. The vasoconstrictor effects of xenogeneic serum on liver circulation are, at least partly, mediated through the release of endothelin by the graft.
Authors:
B Zhang; L Wen; A Gomola; P P Massault; B Cherruau; D Houssin; B Weill; Y Calmus
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  130     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-10     Completed Date:  2000-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  402-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Biologie Cellulaire, Faculté de Médecine Cochin-Port Royal, Université Paris V, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Endothelins / metabolism
Graft Rejection / metabolism*,  physiopathology
Hemodynamics / drug effects
Humans
Immunohistochemistry
Liver / drug effects*,  metabolism,  physiology
Liver Transplantation*
Male
Perfusion
Rats
Rats, Sprague-Dawley
Receptors, Endothelin / antagonists & inhibitors*,  metabolism
Vasoconstrictor Agents / pharmacology*
Chemical
Reg. No./Substance:
0/Endothelins; 0/Receptors, Endothelin; 0/Vasoconstrictor Agents
Comments/Corrections

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