Document Detail


Reversal of enterotoxic diarrhoea by anaesthetic and membrane-stabilizing agents.
MedLine Citation:
PMID:  6295065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of membrane-stabilizing and sedative drugs on enterotoxic diarrhoea was studied in mice. Fluid secretion was induced in ligated loops of the small intestine by challenge with cholera toxin (CT), heat-labile enterotoxin (LT) from Escherichia coli or dibuturyl-cyclic AMP (dB-cAMP). Chlorpromazine, melperone, diazepam, mebumal, ketamine and ethanol all inhibited CT-induced hypersecretion. ED50 being 1.5, 4, 4, 35, 70 and 1500 mg/kg, respectively. The drugs also blocked CT-stimulation of adenylate cyclase, which mediates the action of CT. Concomitant with the antisecretory effect a sedative effect was induced, as judged by the motility and righting reflex of the animals. Hypersecretion by E. coli and LT was also totally blocked by chlorpromazine, diazepam, ketamine and ethanol. In contrast, the secretion by dB-cAMP which bypasses adenylate cyclase in its action, was not affected by diazepam and was only partly reversed by chlorpromazine, ketamine and ethanol. The reversal of dB-cAMP-secretion is probably due to enhanced absorption, rather than inhibition of adenylate-cyclase. The use of membrane-stabilizing drugs may represent a new principle for pharmacological treatment of diarrhoea.
Authors:
I Lönnroth; E Jennische
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica et toxicologica     Volume:  51     ISSN:  0001-6683     ISO Abbreviation:  Acta Pharmacol Toxicol (Copenh)     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370572     Medline TA:  Acta Pharmacol Toxicol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  330-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Anesthetics / pharmacology*
Animals
Antidiarrheals*
Body Fluids / metabolism
Bucladesine / pharmacology
Cholera Toxin / pharmacology
Cyclic AMP / metabolism
Diarrhea / chemically induced
Enterotoxins / antagonists & inhibitors*
Escherichia coli / metabolism
Hypnotics and Sedatives
Male
Mice
Mice, Inbred C57BL
Chemical
Reg. No./Substance:
0/Anesthetics; 0/Antidiarrheals; 0/Enterotoxins; 0/Hypnotics and Sedatives; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; 9012-63-9/Cholera Toxin; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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