| Reversal of altered intestinal mucosal immunity in rats fed elemental diet by supplementation of oleic acid. | |
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MedLine Citation:
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PMID: 8889958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously demonstrated that elemental diet (ED) induces decreased lymphocyte transport in intestinal lymph and significant changes in T cell subsets and the number of IgA-containing cells in gut-associated lymphoid tissues of rats. In order to examine whether the low fat content contributes to the induction of immunological changes in gut-associated lymphoid tissues, the effects of additional fatty acid in the ED were investigated. Rats were divided into four groups: elemental diet alone, elemental diet supplemented with 5% oleic acid (OA), elemental diet with 10% OA and conventional diet as a control. These diets were given at the same daily calorie intake for 4 weeks. The flow rate of intestinal lymph showed no significant difference between the four groups. However, lymphocyte flux as well as the percentage of CD3+ and CD4+ cells were significantly greater in the control and the 10% OA groups than in the ED and 5% OA groups. Intestinal lymph showed decreased concentrations of IgG and IgA in the ED group, whereas the addition of 10% OA significantly attenuated the decrease in these levels. In mesenteric lymph nodes, the CD4+/CD8+ ratio was significantly decreased in the ED group, but 10% OA reversed this change. Immunohistochemical analysis of the ileal mucosa showed that in the ED group the population of CD4+ cells was decreased, while the number of CD8+ cells was increased. Supplementation of OA to ED produced similar stepwise attenuation of the changes in lymphocyte subpopulations in the lamina propria, while the 10% OA group reached levels that were not statistically different from controls. In the elemental diet group, there was a significant decrease in immunoglobulin-containing cells of the IgA class in the lamina propria of the intestine. Similarly, the addition of OA induced dose-dependent recovery in the number of IgA-containing cells. These results suggest that a low dietary concentration of fat may be closely related to changes in lymphocyte transport in intestinal lymph and mucosal immunity of intestinal mucosa induced by the feeding of a long-term ED. |
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Authors:
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H Serizawa; S Miura; H Imaeda; S Tanaka; H Kimura; Y Tsuzuki; H Jing-Yang; K Toda; Y Hamada; M Tsuchiya; H Ishii |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: 11 ISSN: 0815-9319 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1997-01-27 Completed Date: 1997-01-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 811-8 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Kitasato Institute Hospital, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fatty Acids / administration & dosage, pharmacology* Food, Formulated* Ileum / drug effects, immunology Immunity, Mucosal* / drug effects Immunoglobulin A / immunology Lymphoid Tissue / drug effects, immunology* Male Oleic Acid / administration & dosage, pharmacology* Rats Rats, Wistar T-Lymphocyte Subsets / immunology |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Immunoglobulin A; 112-80-1/Oleic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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