Document Detail

Reversal of HCC drug resistance by using hammerhead ribozymes against multidrug resistance 1 gene.
MedLine Citation:
PMID:  16696319     Owner:  NLM     Status:  MEDLINE    
To reverse multidrug resistance(MDR) of HepG2 by anti-MDR1 hammerhead ribozyme, an anti-MDR1 hammerhead ribozyme was developed and delivered to P-gp-overproducing human hepatocarcinoma cell line HepG2 by a retroviral vector containing RNA polymerase III promoter. The expression of mdr1/Pgp and Rz was detected in HepG2, HepG2 multidrug-resistant cell line and HepG2 Rz-transfected cells by semi-quantitative RT-PCR and Western blot methods. Moreover, MTT assay was employed to detect the sensitivity of these ribozyme-transfected cells, and Rhodamine123 (Rh123) was used to test the function of Pgp. The Rz- transfected HepG2 cells became doxorubicin-sensitive, which was concomitant with the decreased MDR1 expression. The study showed that the retrovirus vector encoding the anti-MDR1 ribozyme may be applicable to the treatment of MDR cells.
Sen Qia; Hai Wang; Xiaoping Chen
Related Documents :
10446459 - Lack of modulation of mdr1 gene expression by dominant inhibition of camp-dependent pro...
20621639 - Differences in assessment of macrolide interaction with human mdr1 (abcb1, p-gp) using ...
7908829 - Detection of oligomeric and monomeric forms of p-glycoprotein in multidrug resistant ce...
7902089 - The efflux of anthracyclines in multidrug-resistant cell lines.
9788569 - Lack of correlation of mrp and gamma-glutamylcysteine synthetase overexpression with do...
9863159 - Doxorubicin cellular pharmacokinetics plays no role in chemosensitizing effect of verap...
24859259 - Expression and localization of gonadotropic hormone subunits (gpa, fshb, and lhb) in th...
20167629 - Experimental impact of aspirin exposure on rat intestinal bacteria, epithelial cells an...
22661079 - Changes in morphology, cell wall composition and soluble proteome in rhodobacter sphaer...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  25     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2005  
Date Detail:
Created Date:  2006-05-15     Completed Date:  2007-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  662-4     Citation Subset:  IM    
The Hepatic Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carcinoma, Hepatocellular / drug therapy,  genetics*
Cell Line, Tumor
Drug Resistance, Multiple
Drug Resistance, Neoplasm / genetics*
Liver Neoplasms / drug therapy,  genetics*
P-Glycoprotein / biosynthesis*,  genetics
RNA, Catalytic / genetics*
Reg. No./Substance:
0/P-Glycoprotein; 0/RNA, Catalytic; 0/hammerhead ribozyme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of antisense MBD1 gene eukaryotic expression plasmid on expression of MBD1 gene in human bili...
Next Document:  IL-5 up-regulates the expression of TGF-beta1 in human blood eosinophils in vitro.