Document Detail


Reversal of HCC drug resistance by using hammerhead ribozymes against multidrug resistance 1 gene.
MedLine Citation:
PMID:  16696319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To reverse multidrug resistance(MDR) of HepG2 by anti-MDR1 hammerhead ribozyme, an anti-MDR1 hammerhead ribozyme was developed and delivered to P-gp-overproducing human hepatocarcinoma cell line HepG2 by a retroviral vector containing RNA polymerase III promoter. The expression of mdr1/Pgp and Rz was detected in HepG2, HepG2 multidrug-resistant cell line and HepG2 Rz-transfected cells by semi-quantitative RT-PCR and Western blot methods. Moreover, MTT assay was employed to detect the sensitivity of these ribozyme-transfected cells, and Rhodamine123 (Rh123) was used to test the function of Pgp. The Rz- transfected HepG2 cells became doxorubicin-sensitive, which was concomitant with the decreased MDR1 expression. The study showed that the retrovirus vector encoding the anti-MDR1 ribozyme may be applicable to the treatment of MDR cells.
Authors:
Sen Qia; Hai Wang; Xiaoping Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  25     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2005  
Date Detail:
Created Date:  2006-05-15     Completed Date:  2007-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  662-4     Citation Subset:  IM    
Affiliation:
The Hepatic Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / drug therapy,  genetics*
Cell Line, Tumor
Drug Resistance, Multiple
Drug Resistance, Neoplasm / genetics*
Humans
Liver Neoplasms / drug therapy,  genetics*
P-Glycoprotein / biosynthesis*,  genetics
RNA, Catalytic / genetics*
Chemical
Reg. No./Substance:
0/P-Glycoprotein; 0/RNA, Catalytic; 0/hammerhead ribozyme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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