| Reversal of HCC drug resistance by using hammerhead ribozymes against multidrug resistance 1 gene. | |
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MedLine Citation:
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PMID: 16696319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To reverse multidrug resistance(MDR) of HepG2 by anti-MDR1 hammerhead ribozyme, an anti-MDR1 hammerhead ribozyme was developed and delivered to P-gp-overproducing human hepatocarcinoma cell line HepG2 by a retroviral vector containing RNA polymerase III promoter. The expression of mdr1/Pgp and Rz was detected in HepG2, HepG2 multidrug-resistant cell line and HepG2 Rz-transfected cells by semi-quantitative RT-PCR and Western blot methods. Moreover, MTT assay was employed to detect the sensitivity of these ribozyme-transfected cells, and Rhodamine123 (Rh123) was used to test the function of Pgp. The Rz- transfected HepG2 cells became doxorubicin-sensitive, which was concomitant with the decreased MDR1 expression. The study showed that the retrovirus vector encoding the anti-MDR1 ribozyme may be applicable to the treatment of MDR cells. |
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Authors:
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Sen Qia; Hai Wang; Xiaoping Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban Volume: 25 ISSN: 1672-0733 ISO Abbreviation: J. Huazhong Univ. Sci. Technol. Med. Sci. Publication Date: 2005 |
Date Detail:
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Created Date: 2006-05-15 Completed Date: 2007-08-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101169627 Medline TA: J Huazhong Univ Sci Technolog Med Sci Country: China |
Other Details:
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Languages: eng Pagination: 662-4 Citation Subset: IM |
Affiliation:
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The Hepatic Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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drug therapy,
genetics* Cell Line, Tumor Drug Resistance, Multiple Drug Resistance, Neoplasm / genetics* Humans Liver Neoplasms / drug therapy, genetics* P-Glycoprotein / biosynthesis*, genetics RNA, Catalytic / genetics* |
| Chemical | |
Reg. No./Substance:
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0/P-Glycoprotein; 0/RNA, Catalytic; 0/hammerhead ribozyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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