Document Detail


Reversal of glyphosate inhibition of carrot cell culture growth by glycolytic intermediates and organic and amino acids.
MedLine Citation:
PMID:  16662096     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Various cytokinins and purines were ineffective in reversing glyphosate (0.25 millimolar)-induced growth inhibition of carrot (Daucus carota L.) cell suspension cultures. Aspartate was particularly effective in reversing glyphosate inhibition, but asparagine and various combinations of lysine, methionine, threonine, and homoserine (eventual products of aspartate metabolism) were not effective. When organic acids of the tricarboxylic acid cycle were added to the medium, particularly good reversal of inhibition could be obtained with alpha-ketoglutarate, succinate, and malate. Citrate provided only moderate reversal but the reversal given by glutamate was comparable to that of aspartate and the more effective tricarboxylic acid cycle intermediates. Pyruvate was somewhat toxic to cells when added early in the cell cycle but was most effective at reversing glyphosate inhibition when added at this time. If pyruvate addition was delayed, it was less toxic but was also a less effective reversing agent for glyphosate inhibition.All of the effective reversing agents for glyphosate inhibition found in this study can serve either directly or indirectly as carbon skeletons for respiration and ammonia assimilation and have previously been shown to be effective detoxifying agents for ammonia in cell culture systems. The results of this study suggest that glyphosate inhibition of growth in this system may be due to depletion of respiratory substrate which may eventually result in ammonia accumulation.
Authors:
J Killmer; J Widholm; F Slife
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  68     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1299-302     Citation Subset:  -    
Affiliation:
Department of Agronomy, University of Illinois, Urbana, Illinois 61801.
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