| Revealing the base pair stepping dynamics of nucleic acid motor proteins with optical traps. | |
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MedLine Citation:
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PMID: 20237694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nearly all aspects of nucleic acid metabolism involve motor proteins. This diverse group of enzymes, which includes DNA and RNA polymerases, the ribosome, helicases, and other translocases, converts chemical energy in the form of bond hydrolysis into concerted motion along nucleic acid filaments. The direct observation of this motion at its fundamental distance scale of one base pair has required the development of new ultrasensitive techniques. Recent advances in optical traps have now made these length scales, once the exclusive realm of crystallographic techniques, accessible. Several new studies using optical traps have revealed for the first time how motor proteins translocate along their substrates in a stepwise fashion. Though these techniques have only begun to be applied to biological problems, the unprecedented access into nucleic acid motor protein movement has already provided important insights into their mechanism. In this perspective, we review these advances and offer our view on the future of this exciting development. |
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Authors:
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Yann R Chemla |
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Publication Detail:
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Type: Journal Article Date: 2010-02-19 |
Journal Detail:
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Title: Physical chemistry chemical physics : PCCP Volume: 12 ISSN: 1463-9084 ISO Abbreviation: Phys Chem Chem Phys Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-18 Completed Date: 2010-06-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888160 Medline TA: Phys Chem Chem Phys Country: England |
Other Details:
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Languages: eng Pagination: 3080-95 Citation Subset: IM |
Affiliation:
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Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ychemla@illinois.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Pairing DNA / chemistry* DNA Helicases / chemistry, genetics DNA-Directed RNA Polymerases / chemistry, genetics Kinetics Molecular Motor Proteins / chemistry*, genetics Protein Biosynthesis RNA / chemistry* RNA Helicases / chemistry, genetics Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Molecular Motor Proteins; 63231-63-0/RNA; 9007-49-2/DNA; EC 2.7.7.-/RNA Helicases; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 3.6.1.-/DNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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